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Q88CF3

- DAPF_PSEPK

UniProt

Q88CF3 - DAPF_PSEPK

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Protein

Diaminopimelate epimerase

Gene
dapF, PP_5228
Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Substrate By similarity
Binding sitei46 – 461Substrate By similarity
Binding sitei66 – 661Substrate By similarity
Active sitei75 – 751Proton donor/acceptor By similarity
Binding sitei159 – 1591Substrate By similarity
Sitei161 – 1611Important for catalytic activity By similarity
Binding sitei192 – 1921Substrate By similarity
Sitei210 – 2101Important for catalytic activity By similarity
Active sitei219 – 2191Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-5324-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:PP_5228
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Diaminopimelate epimeraseUniRule annotationPRO_0000149861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 219UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_5228.

Structurei

3D structure databases

ProteinModelPortaliQ88CF3.
SMRiQ88CF3. Positions 3-276.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Substrate binding By similarity
Regioni75 – 773Substrate binding By similarity
Regioni210 – 2112Substrate binding By similarity
Regioni220 – 2212Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiLIVEPPY.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88CF3-1 [UniParc]FASTAAdd to Basket

« Hide

MLLRFTKMHG LGNDFMVLDL VSQHAHIQPK HAKQWGDRHT GVGFDQLLIV    50
EAPNNPEVDF RYRIFNADGS EVEQCGNGAR CFARFVLDKR LTAKKRIRVE 100
TKSGIIVLDV QNDGQVSVDM GPPRFIPAEI PFVADAQALN YPLEVDGQLH 150
SIAAVSMGNP HAVLRVDDVQ TAPVHELGPK IENHPRFPQR VNAGFIQVID 200
RHRANLRVWE RGAGETQACG TGACAAAVAA ISQGWMDSPV SLDLPGGRLH 250
IEWAGPGKPV MMTGPAVRVY EGQVRL 276
Length:276
Mass (Da):30,298
Last modified:June 20, 2003 - v1
Checksum:i4651257E6062A8CB
GO

Sequence cautioni

The sequence AAN70793.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN70793.1. Different initiation.
RefSeqiNP_747329.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN70793; AAN70793; PP_5228.
GeneIDi1042378.
KEGGippu:PP_5228.
PATRICi19949294. VBIPsePut30601_5576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN70793.1 . Different initiation.
RefSeqi NP_747329.1. NC_002947.3.

3D structure databases

ProteinModelPortali Q88CF3.
SMRi Q88CF3. Positions 3-276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160488.PP_5228.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN70793 ; AAN70793 ; PP_5228 .
GeneIDi 1042378.
KEGGi ppu:PP_5228.
PATRICi 19949294. VBIPsePut30601_5576.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi LIVEPPY.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci PPUT160488:GIXO-5324-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiDAPF_PSEPK
AccessioniPrimary (citable) accession number: Q88CF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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