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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83SubstrateUniRule annotation1
Binding sitei97Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-5644-MONOMER
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:PP_5286
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828961 – 151Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST151

Proteomic databases

PRIDEiQ88C95

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_5286

Structurei

3D structure databases

ProteinModelPortaliQ88C95
SMRiQ88C95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Substrate bindingUniRule annotation3
Regioni87 – 89Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z1K Bacteria
COG0756 LUCA
HOGENOMiHOG000028968
KOiK01520
OMAiGVILINH

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00116 dUTPase_bact, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

Q88C95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALQAKILD PRLGSEFPLP AYATPGSAGL DLRALLKEDT ILEPGQTVLI
60 70 80 90 100
PTGLSIYIGD PGLAAVILPR SGLGHKHGIV LGNLVGLIDS DYQGELMVSC
110 120 130 140 150
WNRGNTPFTI AVGERIAQLV LVPVVQAHFE IVEAFDESQR GAGGFGHSGS

H
Length:151
Mass (Da):15,914
Last modified:June 20, 2003 - v1
Checksum:i41BFD14B3CAC9790
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN70851.1
RefSeqiNP_747387.1, NC_002947.4
WP_010955791.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN70851; AAN70851; PP_5286
GeneIDi1042257
KEGGippu:PP_5286
PATRICifig|160488.4.peg.5639

Similar proteinsi

Entry informationi

Entry nameiDUT_PSEPK
AccessioniPrimary (citable) accession number: Q88C95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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