Reviewed, UniProtKB/Swiss-Prot Q88AR2 (ARGA_PSESM)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PSPTO_0324

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186801
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q88AR2-1 [UniParc]. Last modified November 14, 2003. Version 2. Checksum: 705C9A6135D22B57	FASTA	432	47,919
	10 20 30 40 50 60 MPEYVNWLRH ASPYINAHRD CTFVVMLPGD GVAHPNFGNI VHDLVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIESRLAQ RGITPRYHRD MRITDTETLE CVIDAVGQLR ISIEARLSMD MAASPMQGSR 130 140 150 160 170 180 LRVTSGNVVT ARPIGVLEGI DYQHTGEVRR VDRKGINRLL DERHIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVATR AAIDLAADKL LLFGAETGLL DEQGRLVREL RPQQVPAHLQ RLGGNYQAEL 250 260 270 280 290 300 LDAAAEACRG GVARSHIVSY AEDGALLTEL FTRDGGGTLV AQEQFELVRE AAIEDVGGLM 310 320 330 340 350 360 DLITPLEEQG ILVRRSREVL EREITQFSVV EREGLIIACA ALYPIADSES GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIENRAR ALGIKTLFVL TTRTAHWFRE RGFEPSSVDR LPSARASLYN 430 FQRNSKIFEK AI

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO53869.1. Different initiation.
RefSeq	NP_790174.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1181933.
GenomeReviews	Gene locus PSPTO_0324 in contig AE016853_GR.
KEGG	pst:PSPTO_0324.
NMPDR	fig|223283.1.peg.312.
TIGR	PSPTO_0324.
Phylogenomic databases
HOGENOM	Q88AR2.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0324-MON.
BRENDA	2.3.1.1. 289554.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSESM

Accession

Primary (citable) accession number: Q88AR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	November 14, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)