Q88AR1 (GSH1_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	PSPTO_0325

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192534

Sequences

	Sequence		Length	Mass (Da)
	Q88AR1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CA3A6DBC708BC2FA	FASTA	529	59,495
	10 20 30 40 50 60 MSELLNRRLA LLGERNNLSL LEQCLHGIER ECLRVTPQAE LARTPHPQAL GAALTNGLVT 70 80 90 100 110 120 TDYSESLLEF ITPALKNPVD TLDSLDKIHR FAYSKLDNEL LWSPSMPCPL PDEEHIPIAY 130 140 150 160 170 180 YGTSNIGKLK YVYRKGLALR YGKTMQCIAG IHYNFSLPED AWALLKQTED FAGDARDYQS 190 200 210 220 230 240 HSYIALIRNF RRYSWLLMYL FGASPALDAG FLRGREHQLE QFDADTLYLP YATSLRMSDL 250 260 270 280 290 300 GYQSDAQADL TPCYNDLVSY TDSLRKAVAT PYKPYVDIGT HDQNGEWVQL NTNVLQIENE 310 320 330 340 350 360 YYSNIRPKRV TYSGERPIQA LVARGVQYVE VRCLDINPFL PTGISLEQSR FIDAFVLYCA 370 380 390 400 410 420 LEESQQLAGH ECSNASSNFL AVVKEGRRPG LNLQRDDSPV ELKAWATDLL EKITPIARLL 430 440 450 460 470 480 DQAQGTDEHI KSIAVQQAKI DDTSLTPSAQ VLASMRAHGE GFTAFSLRQS QVHAEYFRTH 490 500 510 520 PLSAAEQAHF EDLAKTSLEE QAELEATEEV VDFDTFVGSY QASILSISN

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO53870.1.
RefSeq	NP_790175.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q88AR1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1181934.
GenomeReviews	Gene locus PSPTO_0325 in contig AE016853_GR.
KEGG	pst:PSPTO_0325.
NMPDR	fig|223283.1.peg.313.
PATRIC	19991826. VBIPseSyr93040_0341.
TIGR	PSPTO_0325.
Phylogenomic databases
HOGENOM	HBG289023.
OMA	ADTLYLP.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0325-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PSESM

Accession

Primary (citable) accession number: Q88AR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program