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Q88AF5

- TRMB_PSESM

UniProt

Q88AF5 - TRMB_PSESM

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (16 Jun 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
    Binding sitei99 – 991S-adenosyl-L-methionineUniRule annotation
    Binding sitei126 – 1261S-adenosyl-L-methionineUniRule annotation
    Active sitei149 – 1491By similarity
    Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation
    Binding sitei185 – 1851SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-431-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:PSPTO_0435
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 244244tRNA (guanine-N(7)-)-methyltransferasePRO_0000171378Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi223283.PSPTO_0435.

    Structurei

    3D structure databases

    ProteinModelPortaliQ88AF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni222 – 2254Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.
    PhylomeDBiQ88AF5.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q88AF5-1 [UniParc]FASTAAdd to Basket

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    MTDTHVPPPE LPAAEEGEER PHRRIKSFVM RAGRMTEGQQ RGLDQGRPLF    50
    GLSLTDTPVD FDQVFGRSAP RTLEIGFGMG HSLLEMAAAS PEQDFIGVEV 100
    HYPGVGAMLN GVLTQGLTNV RVYDCDAIEV LNRCIADNSL DRLMLFFPDP 150
    WHKSRHHKRR IVQPEFAALV RSKLKVGGVF HMATDWGPYA EYMLEVMSVA 200
    PGYRNQAEDN QYVPRPAERP ITKFERRGEK LGHGVWDLKF EKVD 244
    Length:244
    Mass (Da):27,569
    Last modified:June 16, 2003 - v1
    Checksum:i05DA6C15DBA800A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO53979.1.
    RefSeqiNP_790284.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO53979; AAO53979; PSPTO_0435.
    GeneIDi1182044.
    KEGGipst:PSPTO_0435.
    PATRICi19992054. VBIPseSyr93040_0455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO53979.1 .
    RefSeqi NP_790284.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q88AF5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_0435.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO53979 ; AAO53979 ; PSPTO_0435 .
    GeneIDi 1182044.
    KEGGi pst:PSPTO_0435.
    PATRICi 19992054. VBIPseSyr93040_0455.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.
    PhylomeDBi Q88AF5.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PSYR223283:GJIX-431-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.

    Entry informationi

    Entry nameiTRMB_PSESM
    AccessioniPrimary (citable) accession number: Q88AF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3