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Q88AF5

- TRMB_PSESM

UniProt

Q88AF5 - TRMB_PSESM

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Binding sitei99 – 991S-adenosyl-L-methionineUniRule annotation
Binding sitei126 – 1261S-adenosyl-L-methionineUniRule annotation
Active sitei149 – 1491By similarity
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-431-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PSPTO_0435
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244tRNA (guanine-N(7)-)-methyltransferasePRO_0000171378Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_0435.

Structurei

3D structure databases

ProteinModelPortaliQ88AF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2254Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.
PhylomeDBiQ88AF5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88AF5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDTHVPPPE LPAAEEGEER PHRRIKSFVM RAGRMTEGQQ RGLDQGRPLF
60 70 80 90 100
GLSLTDTPVD FDQVFGRSAP RTLEIGFGMG HSLLEMAAAS PEQDFIGVEV
110 120 130 140 150
HYPGVGAMLN GVLTQGLTNV RVYDCDAIEV LNRCIADNSL DRLMLFFPDP
160 170 180 190 200
WHKSRHHKRR IVQPEFAALV RSKLKVGGVF HMATDWGPYA EYMLEVMSVA
210 220 230 240
PGYRNQAEDN QYVPRPAERP ITKFERRGEK LGHGVWDLKF EKVD
Length:244
Mass (Da):27,569
Last modified:June 16, 2003 - v1
Checksum:i05DA6C15DBA800A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO53979.1.
RefSeqiNP_790284.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO53979; AAO53979; PSPTO_0435.
GeneIDi1182044.
KEGGipst:PSPTO_0435.
PATRICi19992054. VBIPseSyr93040_0455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO53979.1 .
RefSeqi NP_790284.1. NC_004578.1.

3D structure databases

ProteinModelPortali Q88AF5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223283.PSPTO_0435.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO53979 ; AAO53979 ; PSPTO_0435 .
GeneIDi 1182044.
KEGGi pst:PSPTO_0435.
PATRICi 19992054. VBIPseSyr93040_0455.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.
PhylomeDBi Q88AF5.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci PSYR223283:GJIX-431-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiTRMB_PSESM
AccessioniPrimary (citable) accession number: Q88AF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3