Reviewed, UniProtKB/Swiss-Prot Q88AE7 (BETA_PSESM)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	PSPTO_0443

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Choline dehydrogenase HAMAP MF_00750		PRO_0000205592
Regions
	Nucleotide binding	8 – 37	30	FAD Probable
Sites
	Active site	477	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88AE7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 147045C3DDCC9005	FASTA	568	62,788
	10 20 30 40 50 60 MTTQSEYDYI IIGAGSAGNT LAARLTEDAG VTVLLLEAGG PDYRLDFRTQ MPAALAFPLQ 70 80 90 100 110 120 GRRYNWAYET EPEPHMDNRR MECGRGKGLG GSSLINGMCY IRGNAMDYDG WAKEPGLEDW 130 140 150 160 170 180 SYLDCLPYFR KAETRDIGPN DYHGGEGPVS VTTPKAGNNP LFHAMVEAGV QAGFPRTDDL 190 200 210 220 230 240 NGYQQEGFGP MDRTVTPKGR RASTARGYLD EAKKRDTLSI VTHALTDRIL FEGKRAVGVA 250 260 270 280 290 300 YLVGDSDTRI EVRARKEVLL CGGAIASPQI LQRSGVGPAE VLNKLDIPVV HDLPGVGQNL 310 320 330 340 350 360 QDHLEMYLQY ACTQPVSLYP SLKWWNQPAI GAEWMFLGTG IGASNQFEAG GFIRSSEAFE 370 380 390 400 410 420 WPNIQYHFLP VAINYNGTKG VQEHGFQAHV GSMRSPSRGR VHVKSKDPRE YPSILFNYMA 430 440 450 460 470 480 SDQDWQEFRD GIRLTREIMQ QPALDPYRGR EISPGIDVQS DEALDQFVRE HAETAYHPSC 490 500 510 520 530 540 SCKMGTDEMA VVDGQGRVHG MQNLRVVDAS IMPIITTGNL NAPTIMIAEK IADKIRGRQP 550 560 LPRSTADYFV AGDKPARGKP LREISHQA

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO53987.1.
RefSeq	NP_790292.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1182052.
GenomeReviews	Gene locus PSPTO_0443 in contig AE016853_GR.
KEGG	pst:PSPTO_0443.
NMPDR	fig|223283.1.peg.430.
TIGR	PSPTO_0443.
Phylogenomic databases
HOGENOM	Q88AE7.
OMA	PNLQYHF.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0443-MON.
BRENDA	1.1.99.1. 289554.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSESM

Accession

Primary (citable) accession number: Q88AE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)