Reviewed, UniProtKB/Swiss-Prot Q88AD1 (GLYA1_PSESM)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Synonyms:	glyA-1
Ordered Locus Names:	PSPTO_0461

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000113642
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88AD1-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: C7969A7044DE387B	FASTA	417	44,875
	10 20 30 40 50 60 MFSKQDQIQG YDDALLSAMN AEEQRQEDHI ELIASENYTS KRVMQAQGSG LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEH VDKVEQLAIE RARQLFGADY ANVQPHSGSQ ANAAVYLALL QAGDTVLGMS 130 140 150 160 170 180 LAHGGHLTHG AKVSFSGKLY NAVQYGIDTT TGLIDYDEVE RIAVECQPKM LIAGFSAYSK 190 200 210 220 230 240 TLDFPRFRAI ADKVGAYLFV DMAHVAGLVA AGLYPNPLPY ADVVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LAKANEELEK KFNSAVFPGG QGGPLMHVIA AKAVCFKEAM EPGFKTYQQQ VIDNAQAMAQ 310 320 330 340 350 360 VFITRGFDVV SGGTDNHLFL VSLIRQGLTG KEADAALGRA HITVNKNSVP NDPQSPFVTS 370 380 390 400 410 GLRIGTPAVT TRGFKVTQCI ELAGWICDIL DNLGAADVEA NVASQVAALC ADFPVYR

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO54005.1.
RefSeq	NP_790310.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
SMR	Q88AD1. Positions 1-416.
ModBase	Search...
Genome annotation databases
GeneID	1182070.
GenomeReviews	Gene locus PSPTO_0461 in contig AE016853_GR.
KEGG	pst:PSPTO_0461.
NMPDR	fig|223283.1.peg.448.
TIGR	PSPTO_0461.
Phylogenomic databases
HOGENOM	Q88AD1.
OMA	QVIDNAQ.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0461-MON.
BRENDA	2.1.2.1. 289554.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_PSESM

Accession

Primary (citable) accession number: Q88AD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)