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Protein

Malate synthase G 1

Gene

glcB1

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei631 – 6311Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-477-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G 1UniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcB1UniRule annotation
Synonyms:glcB-1
Ordered Locus Names:PSPTO_0480
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase G 1PRO_0000166894Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_0480.

Structurei

3D structure databases

ProteinModelPortaliQ88AB2.
SMRiQ88AB2. Positions 2-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.
PhylomeDBiQ88AB2.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88AB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYVQVGDL QVARVLFDFV QNEATPGTGV DAGAFWAGAD QLIHDLAPKN
60 70 80 90 100
KALLAQRDEL QAQIDAWHQS RAGQAHDASA YKAFLQEIGY LLPEAADFQI
110 120 130 140 150
TTQNVDEEIA TMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE
160 170 180 190 200
EGGAEKGKGY NKVRGDKVIA FARAFLDQAA PLAAGSHVDS TAYKLIDGRL
210 220 230 240 250
VISLKGGSNT GLRDDAQLVG FQGDASAPFA VLFKHNGLHF ELQIDAASPV
260 270 280 290 300
GQTDPAGVKD IVMEAALTTI MDCEDSIAAV DADDKVVVYR NWLGLMKGDL
310 320 330 340 350
VESVSKGGET FTRTMNPDRV YTTPQGGEVT LHGRSLLFIR NVGHLMTIDA
360 370 380 390 400
ILDKHGNEVP EGILDGLLTS LAAIHNLNGN NTRSNSRSGS MYIVKPKMHG
410 420 430 440 450
PQEAAFTNEL FGRIEQVLGL PRNTLKVGIM DEERRTTVNL KACIQAASER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGPVVRKAQ MKAEKWISAY ENSNVDIGLK
510 520 530 540 550
CGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA
560 570 580 590 600
LHYHKVDVFA RQAELAQREQ ASVDDILTIP LALNTDWTPE QVQNELDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDIGLME DRATLRISSQ HMTNWLRHGI
660 670 680 690 700
VTETQVLESL KRMAPVVDRQ NANDPLYRPL SPDFDSNIAF QAAVELVIEG
710 720
TRQPNGYTEP VLHRRRREFK AKNRV
Length:725
Mass (Da):79,144
Last modified:April 30, 2003 - v1
Checksum:iF1993264E8083660
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO54024.1.
RefSeqiNP_790329.1. NC_004578.1.
WP_005763731.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO54024; AAO54024; PSPTO_0480.
GeneIDi1182089.
KEGGipst:PSPTO_0480.
PATRICi19992138. VBIPseSyr93040_0497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO54024.1.
RefSeqiNP_790329.1. NC_004578.1.
WP_005763731.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ88AB2.
SMRiQ88AB2. Positions 2-722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_0480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO54024; AAO54024; PSPTO_0480.
GeneIDi1182089.
KEGGipst:PSPTO_0480.
PATRICi19992138. VBIPseSyr93040_0497.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.
PhylomeDBiQ88AB2.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciPSYR223283:GJIX-477-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiMASZ1_PSESM
AccessioniPrimary (citable) accession number: Q88AB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.