Q88AB2 (MASZ1_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G 1
EC=2.3.3.9

Gene names

Name:	glcB1
Synonyms:	glcB-1
Ordered Locus Names:	PSPTO_0480

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G 1 HAMAP MF_00641		PRO_0000166894
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88AB2 [UniParc]. Last modified April 30, 2003. Version 1. Checksum: F1993264E8083660	FASTA	725	79,144
	10 20 30 40 50 60 MTEYVQVGDL QVARVLFDFV QNEATPGTGV DAGAFWAGAD QLIHDLAPKN KALLAQRDEL 70 80 90 100 110 120 QAQIDAWHQS RAGQAHDASA YKAFLQEIGY LLPEAADFQI TTQNVDEEIA TMAGPQLVVP 130 140 150 160 170 180 VMNARFALNA SNARWGSLYD ALYGTDAISE EGGAEKGKGY NKVRGDKVIA FARAFLDQAA 190 200 210 220 230 240 PLAAGSHVDS TAYKLIDGRL VISLKGGSNT GLRDDAQLVG FQGDASAPFA VLFKHNGLHF 250 260 270 280 290 300 ELQIDAASPV GQTDPAGVKD IVMEAALTTI MDCEDSIAAV DADDKVVVYR NWLGLMKGDL 310 320 330 340 350 360 VESVSKGGET FTRTMNPDRV YTTPQGGEVT LHGRSLLFIR NVGHLMTIDA ILDKHGNEVP 370 380 390 400 410 420 EGILDGLLTS LAAIHNLNGN NTRSNSRSGS MYIVKPKMHG PQEAAFTNEL FGRIEQVLGL 430 440 450 460 470 480 PRNTLKVGIM DEERRTTVNL KACIQAASER VVFINTGFLD RTGDEIHTSM EAGPVVRKAQ 490 500 510 520 530 540 MKAEKWISAY ENSNVDIGLK CGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV 550 560 570 580 590 600 PSPTAAALHA LHYHKVDVFA RQAELAQREQ ASVDDILTIP LALNTDWTPE QVQNELDNNA 610 620 630 640 650 660 QGILGYVVRW IDQGVGCSKV PDINDIGLME DRATLRISSQ HMTNWLRHGI VTETQVLESL 670 680 690 700 710 720 KRMAPVVDRQ NANDPLYRPL SPDFDSNIAF QAAVELVIEG TRQPNGYTEP VLHRRRREFK AKNRV

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO54024.1.
RefSeq	NP_790329.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q88AB2.
SMR	Q88AB2. Positions 2-722.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1182089.
GenomeReviews	Gene locus PSPTO_0480 in contig AE016853_GR.
KEGG	pst:PSPTO_0480.
NMPDR	fig|223283.1.peg.467.
PATRIC	19992138. VBIPseSyr93040_0497.
TIGR	PSPTO_0480.
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0480-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ1_PSESM

Accession

Primary (citable) accession number: Q88AB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program