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Q88AB2

- MASZ1_PSESM

UniProt

Q88AB2 - MASZ1_PSESM

Protein

Malate synthase G 1

Gene

glcB1

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-477-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase G 1UniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcB1UniRule annotation
    Synonyms:glcB-1
    Ordered Locus Names:PSPTO_0480
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase G 1PRO_0000166894Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi223283.PSPTO_0480.

    Structurei

    3D structure databases

    ProteinModelPortaliQ88AB2.
    SMRiQ88AB2. Positions 2-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.
    PhylomeDBiQ88AB2.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q88AB2-1 [UniParc]FASTAAdd to Basket

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    MTEYVQVGDL QVARVLFDFV QNEATPGTGV DAGAFWAGAD QLIHDLAPKN    50
    KALLAQRDEL QAQIDAWHQS RAGQAHDASA YKAFLQEIGY LLPEAADFQI 100
    TTQNVDEEIA TMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 150
    EGGAEKGKGY NKVRGDKVIA FARAFLDQAA PLAAGSHVDS TAYKLIDGRL 200
    VISLKGGSNT GLRDDAQLVG FQGDASAPFA VLFKHNGLHF ELQIDAASPV 250
    GQTDPAGVKD IVMEAALTTI MDCEDSIAAV DADDKVVVYR NWLGLMKGDL 300
    VESVSKGGET FTRTMNPDRV YTTPQGGEVT LHGRSLLFIR NVGHLMTIDA 350
    ILDKHGNEVP EGILDGLLTS LAAIHNLNGN NTRSNSRSGS MYIVKPKMHG 400
    PQEAAFTNEL FGRIEQVLGL PRNTLKVGIM DEERRTTVNL KACIQAASER 450
    VVFINTGFLD RTGDEIHTSM EAGPVVRKAQ MKAEKWISAY ENSNVDIGLK 500
    CGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 550
    LHYHKVDVFA RQAELAQREQ ASVDDILTIP LALNTDWTPE QVQNELDNNA 600
    QGILGYVVRW IDQGVGCSKV PDINDIGLME DRATLRISSQ HMTNWLRHGI 650
    VTETQVLESL KRMAPVVDRQ NANDPLYRPL SPDFDSNIAF QAAVELVIEG 700
    TRQPNGYTEP VLHRRRREFK AKNRV 725
    Length:725
    Mass (Da):79,144
    Last modified:April 30, 2003 - v1
    Checksum:iF1993264E8083660
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO54024.1.
    RefSeqiNP_790329.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO54024; AAO54024; PSPTO_0480.
    GeneIDi1182089.
    KEGGipst:PSPTO_0480.
    PATRICi19992138. VBIPseSyr93040_0497.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO54024.1 .
    RefSeqi NP_790329.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q88AB2.
    SMRi Q88AB2. Positions 2-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_0480.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO54024 ; AAO54024 ; PSPTO_0480 .
    GeneIDi 1182089.
    KEGGi pst:PSPTO_0480.
    PATRICi 19992138. VBIPseSyr93040_0497.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.
    PhylomeDBi Q88AB2.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci PSYR223283:GJIX-477-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.

    Entry informationi

    Entry nameiMASZ1_PSESM
    AccessioniPrimary (citable) accession number: Q88AB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3