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Q88A98

- BIOB_PSESM

UniProt

Q88A98 - BIOB_PSESM

Protein

Biotin synthase

Gene

bioB

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi103 – 1031Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-491-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:PSPTO_0494
    ORF Names:PSPTO0494
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352Biotin synthasePRO_0000381565Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi223283.PSPTO_0494.

    Structurei

    3D structure databases

    ProteinModelPortaliQ88A98.
    SMRiQ88A98. Positions 10-319.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.
    PhylomeDBiQ88A98.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q88A98-1 [UniParc]FASTAAdd to Basket

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    MSASTTATLR HDWTLAEVRA LFVQPFNDLL FQAQTLHRAH FDANRVQVST    50
    LLSIKTGACP EDCKYCPQSG HYNTGLEKEK LLEVQKVLEE AARAKSIGST 100
    RFCMGAAWKH PSAKDMPYVL EMVKGVKAMG LETCMTLGRL DQEQTEALAT 150
    AGLDYYNHNL DTSPEFYGSI ITTRTYGERL QTLAYVRDAG MKICSGGILG 200
    MGESLDDRAG LLIQLANLPE HPESVPINML VKVAGTPLEN AEDVDPFDFI 250
    RMLAVARILM PQSHVRLSAG REAMNEQMQA LAFFAGANSI FYGDKLLTTA 300
    NPQADKDMLL FSRLGIKPEA GEGHADEVHQ AAIEQALVEQ QSSAMFYDAA 350
    SA 352
    Length:352
    Mass (Da):38,610
    Last modified:June 1, 2003 - v1
    Checksum:iB2AB050D00F03F50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO54038.1.
    RefSeqiNP_790343.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO54038; AAO54038; PSPTO_0494.
    GeneIDi1182103.
    KEGGipst:PSPTO_0494.
    PATRICi19992166. VBIPseSyr93040_0511.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO54038.1 .
    RefSeqi NP_790343.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q88A98.
    SMRi Q88A98. Positions 10-319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_0494.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO54038 ; AAO54038 ; PSPTO_0494 .
    GeneIDi 1182103.
    KEGGi pst:PSPTO_0494.
    PATRICi 19992166. VBIPseSyr93040_0511.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.
    PhylomeDBi Q88A98.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci PSYR223283:GJIX-491-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.

    Entry informationi

    Entry nameiBIOB_PSESM
    AccessioniPrimary (citable) accession number: Q88A98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3