Reviewed, UniProtKB/Swiss-Prot Q889M7 (IPYR1_PSESM)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase 1
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase 1
      Short name=PPase 1

Gene names

Name:	ppa1
Ordered Locus Names:	PSPTO_0722

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Inorganic pyrophosphatase 1 HAMAP MF_00209		PRO_0000137521
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q889M7-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 70A62F7AB54D49E6	FASTA	175	19,445
	10 20 30 40 50 60 MSYSKIPAGK DLPNDIYVAI EIPANHAPIK YEIDKDTDCL FVDRFMATPM FYPANYGFIP 70 80 90 100 110 120 NTLADDGDPL DVLVVTPYPV TPGSVIRARP VGILHMTDDG GGDAKVIAVP HDKLSQLYVD 130 140 150 160 170 VKEYTDLPAL LLEQIKHFFE NYKDLEKGKW VKIEGWGNAD AARAEIMKSV EAFKG

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO54264.1.
RefSeq	NP_790569.1.
3D structure databases
HSSP	HSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBase	Search...
Genome annotation databases
GeneID	1182342.
GenomeReviews	Gene locus PSPTO_0722 in contig AE016853_GR.
KEGG	pst:PSPTO_0722.
NMPDR	fig|223283.1.peg.707.
TIGR	PSPTO_0722.
Phylogenomic databases
HOGENOM	Q889M7.
OMA	ELDVLVY.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_0722-MON.
BRENDA	3.6.1.1. 289554.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR1_PSESM

Accession

Primary (citable) accession number: Q889M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)