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Protein

Replicase large subunit

Gene
N/A
Organism
Turnip vein-clearing virus (TVCV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarityCurated

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon which is expressed as tyrosine. When readthrough of the terminator codon TGA occurs between the codons for Phe-1116 and Asp-1118, this results in the addition of the RdRp region to the replicase.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi827 – 834ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiTurnip vein-clearing virus (TVCV)
Taxonomic identifieri29272 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiBrassicaceae [TaxID: 3700]
Proteomesi
  • UP000008264 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000411981 – 1601Replicase large subunitAdd BLAST1601
ChainiPRO_00000411991 – 1107Replicase small subunitAdd BLAST1107

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ88920.
SMRiQ88920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 280Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST209
Domaini795 – 954(+)RNA virus helicase ATP-bindingAdd BLAST160
Domaini955 – 1107(+)RNA virus helicase C-terminalAdd BLAST153
Domaini1369 – 1482RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 436MethyltransferaseAdd BLAST387
Regioni823 – 1076HelicaseAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000048.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q88920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFQQTIDM QTLQAAAGRN SLVNDLASRR VYDNAVEELN ARSRRPKVHF
60 70 80 90 100
SKAVSTEQTL IATNAYPEFE ISFTHTQSAV HSLAGGLRSL ELEYLMMQVP
110 120 130 140 150
FGSLTYDIGG NFSAHLFKGR DYVHCCMPNL DVRDIARHEG HKEAIYSYVN
160 170 180 190 200
RLKRQQRPVP EYQRAAFNNY AENPHFVHCD KPFQQCELTT AYGTDTYAVA
210 220 230 240 250
LHSIYDIPVE EFGSALLRKN VKTCFAAFHF HENMLLDCDT VTLDEIGATF
260 270 280 290 300
QKSGDNLSFF FHNESTLNYT HSFSNIIKYV CKTFFPASQR FVYHKEFLVT
310 320 330 340 350
RVNTWYCKFT RVDTFTLFRG VYHNNVDCEE FYKAMDDAWH YKKTLAMLNA
360 370 380 390 400
ERTIFKDNAA LNFWFPKVRD MVIVPLFDAS ITTGRMSRRE IMVNKDFVYT
410 420 430 440 450
VLNHIKTYQA KALTYANVLS FVESIRSRVI INGVTARSEW DTDKAILGPL
460 470 480 490 500
AMTFFLITKL GHVQDEIILK KFQKFDRTTN ELIWTSLCDA LMGVIPSVKE
510 520 530 540 550
TLVRGGFVKV AEQALEIKVP ELYCTFADRL VLQYKKAEEF QSCDLSKPLE
560 570 580 590 600
ESEKYYNALS ELSVLENLDS FDLEAFKTLC QQKNVDPDMA AKVVVAIMKS
610 620 630 640 650
ELTLPFKKPT EEEISESLKP GEGSCAEHKE VLSLQNDAPF PCVKNLVEGS
660 670 680 690 700
VPAYGMCPKG GGFDKLDVDI ADFHLKSVDA VKKGTMMSAV YTGSIKVQQM
710 720 730 740 750
KNYIDYLSAS LAATVSNLCK VLRDVHGVDP ESQEKSGVWD VRRGRWLLKP
760 770 780 790 800
NAKSHAWGVA EDANHKLVIV LLNWDDGKPV CDETWFRVAV SSDSLIYSDM
810 820 830 840 850
GKLKTLTSCS PNGEPPEPNA KVILVDGVPG CGKTKEIIEK VNFSEDLILV
860 870 880 890 900
PGKEASKMII RRANQAGVIR ADKDNVRTVD SFLMHPSRRV FKRLFIDEGL
910 920 930 940 950
MLHTGCVNFL LLLSQCDVAY VYGDTKQIPF ICRVANFPYP AHFAKLVADE
960 970 980 990 1000
KEVRRVTLRC PADVTYFLNK KYDGAVMCTS AVERSVKAEV VRGKGALNPI
1010 1020 1030 1040 1050
TLPLEGKILT FTQADKFELL EKGYKDVNTV HEVQGETYEK TAIVRLTSTP
1060 1070 1080 1090 1100
LEIISSASPH VLVALTRHTT CCKYYTVVLD PMVNVISEME KLSNFLLDMY
1110 1120 1130 1140 1150
RVEAGVQYQL QIDAVFRDSN LFVQTPKSGD WRDMQFYYDA LLPGNSTILN
1160 1170 1180 1190 1200
EFDAVTMNLR DISLNVKDCR IDFSKSVQLP KEQPIFLKPK IRTAAEMPRT
1210 1220 1230 1240 1250
AGLLENLVAM IKRNMNAPDL TGTIDIEDTA SLVVEKFWDS YVDKEFSGTN
1260 1270 1280 1290 1300
EMTMTRESFS RWLSKQESST VGQLADFNFV DLPAVDEYKH MIKSQPKQKL
1310 1320 1330 1340 1350
DLSIQDEYPA LQTIVYHSKK INAIFGPMFS ELTRMLLERI DSSKFLFYTR
1360 1370 1380 1390 1400
KTPAQIEDFF SDLDSTQAME ILELDISKYD KSQNEFHCAV EYKIWEKLGI
1410 1420 1430 1440 1450
DEWLAEVWKQ GHRKTTLKDY TAGVKTCLWY QRKSGDVTTF IGNTIIIAAC
1460 1470 1480 1490 1500
LSSMIPMDKV IKAAFCGDDS LIYIPKGLDL PDIQAGANLM WNFEAKLFRK
1510 1520 1530 1540 1550
KYGYFCGRYV IHHDRGAIVY YDPLKLISKL GCKHIRDVVH LEELRESLCD
1560 1570 1580 1590 1600
VASNLNNCAY FSQLDEAVAE VHKTAVGGSF AFCSIIKYLS DKRLFRDLFF

V
Length:1,601
Mass (Da):181,930
Last modified:November 1, 1996 - v1
Checksum:iF9E0A041C15DB34D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03387 Genomic RNA. Translation: AAC02783.1.
U03387 Genomic RNA. Translation: AAC02782.1.
RefSeqiNP_046151.1. NC_001873.1.
NP_046152.1. NC_001873.1.

Genome annotation databases

GeneIDi1494923.
1494926.
KEGGivg:1494923.
vg:1494926.

Keywords - Coding sequence diversityi

RNA suppression of termination

Similar proteinsi

Entry informationi

Entry nameiRDRP_TVCV
AccessioniPrimary (citable) accession number: Q88920
Secondary accession number(s): Q88919
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families