Q887Q9 (ALGA_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate biosynthesis protein AlgA

Including the following 2 domains:

1. Mannose-6-phosphate isomerase
   EC=5.3.1.8
   Alternative name(s):
   Phosphohexomutase
   Phosphomannose isomerase
   Short name=PMI
2. Mannose-1-phosphate guanylyltransferase
   EC=2.7.7.13
   Alternative name(s):
   GDP-mannose pyrophosphorylase
   Short name=GMP
   Short name=GMPP
   GTP--mannose-1-phosphate guanylyltransferase

Gene names

Name:	algA
Ordered Locus Names:	PSPTO_1232

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 3 › Pseudomonas syringae pv. tomato

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate. GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Cofactor	Cobalt (for PMI) By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Alginate biosynthesis
Ligand	Cobalt GTP-binding Nucleotide-binding
Molecular function	Isomerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway alginic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Alginate biosynthesis protein AlgA		PRO_0000194251

Sequences

	Sequence		Length	Mass (Da)
	Q887Q9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B9EFCA39C452FE44	FASTA	483	53,563
	10 20 30 40 50 60 MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL QRLVFEGMQE PIVVCNKDHR 70 80 90 100 110 120 FIVNEQLAAL NLETQAILME PFGRNTAPAV ALTAMKLVNE GNDGLMLVLP ADHVIEDQKA 130 140 150 160 170 180 LQRALALATV TAERGEMVLF GVPANKPETG YGYIKSTADA LLPEGVSRVS QFVEKPDEKR 190 200 210 220 230 240 AKEFVEAGGY YWNSGMFLFR ASRFLEELKK HDPDIYDTCL LTLERSVQDG DALEIDASTF 250 260 270 280 290 300 ACCPDNSIDY AVMEKTQRAC VVPLSAGWSD VGCWSSLWEV NAKDAHGNVT KGDVVIQDSR 310 320 330 340 350 360 NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVATLNEQG RTETQNHLEV 370 380 390 400 410 420 YRPWGSYDSV DMGGRFQVKR ISVKPGACLS LQMHHHRAEH WIVVSGTAQV TCDENVFLLT 430 440 450 460 470 480 ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS GSYLGEDDIE RFEDIYGRSN ALEAGVKTQT IAR

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO54757.1.
RefSeq	NP_791062.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q887Q9.
ModBase	Search...
Protein-protein interaction databases
STRING	223283.PSPTO_1232.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO54757; AAO54757; PSPTO_1232.
GeneID	1182868.
KEGG	pst:PSPTO_1232.
PATRIC	19993704. VBIPseSyr93040_1253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0662.
HOGENOM	HOG000153549.
KO	K16011.
OMA	ASTFACC.
ProtClustDB	CLSK868066.
Enzyme and pathway databases
BioCyc	PSYR223283:GJIX-1257-MONOMER.
UniPathway	UPA00126; UER00423. UPA00126; UER00930.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGA_PSESM

Accession

Primary (citable) accession number: Q887Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program