Reviewed, UniProtKB/Swiss-Prot Q887Q5 (ALGL_PSESM)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PSPTO_1236

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 378	350	Alginate lyase HAMAP MF_00557		PRO_0000024921

Sequences

	Sequence		Length	Mass (Da)
	Q887Q5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E5961DC8BD67A487	FASTA	378	42,486
	10 20 30 40 50 60 MQTPKLIRPT LLSMAIVSSM AWATGASAAL VPPKGYDAPI EKMKTGDHNF TCEAIPKPYT 70 80 90 100 110 120 DKLVFRSKYE GSDKARATLN AVSEEAFRDA TKDITTLERG VSKVVMQYMR DGRPEQLDCA 130 140 150 160 170 180 LNMMTTWAKA DALESREFNH TGKSMRKWAL GSMSSAYLRL KFSDSHPLAN RQQDAQIIEA 190 200 210 220 230 240 WFSKLADQVV SDWSNLPLEK INNHSYWAAW SVMSTAVVTN RKDLFDWAVK EFKVAANQVD 250 260 270 280 290 300 KDGYLPNEMK RRQRALSYHN YALPPLAMIA SFAQANGVDL RPENNGALKR LGDRVLAGVK 310 320 330 340 350 360 DPASFAEHNG EKQDMTDLKK DPKFAWLEPY CSLYTCSPDV LEDKHEKQPF KTFRLGGDLT 370 KVYDPTHEKG DKGDNDGS

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO54761.1.
RefSeq	NP_791066.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1182872.
GenomeReviews	Gene locus PSPTO_1236 in contig AE016853_GR.
KEGG	pst:PSPTO_1236.
NMPDR	fig|223283.1.peg.1204.
TIGR	PSPTO_1236.
Phylogenomic databases
HOGENOM	HBG336064.
OMA	RSKYEGS.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_1236-MONOMER.
BRENDA	4.2.2.3. 289554.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR008397. Alginate_lyase. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSESM

Accession

Primary (citable) accession number: Q887Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)