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Protein

Poly(beta-D-mannuronate) C5 epimerase

Gene

algG

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional protein that converts poly(beta-D-mannuronate) to alpha-L-guluronate and that is also part of a periplasmic protein complex that serves as a scaffold that leads the newly formed alginate polymer through the periplasmic space to the outer membrane secretin AlgE.By similarity

Pathwayi: alginate biosynthesis

This protein is involved in the pathway alginate biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway alginate biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Alginate biosynthesis

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-1263-MONOMER.
UniPathwayiUPA00286.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(beta-D-mannuronate) C5 epimerase (EC:5.1.3.-)
Gene namesi
Name:algG
Ordered Locus Names:PSPTO_1238
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Sequence analysisAdd
BLAST
Chaini37 – 536500Poly(beta-D-mannuronate) C5 epimerasePRO_0000001128Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1238.

Structurei

Secondary structure

1
536
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi83 – 875Combined sources
Beta strandi93 – 953Combined sources
Beta strandi97 – 1026Combined sources
Helixi103 – 1053Combined sources
Helixi107 – 1137Combined sources
Helixi116 – 1183Combined sources
Helixi120 – 1267Combined sources
Beta strandi131 – 14111Combined sources
Helixi142 – 1487Combined sources
Turni151 – 1533Combined sources
Beta strandi154 – 1585Combined sources
Beta strandi161 – 1644Combined sources
Beta strandi168 – 1703Combined sources
Beta strandi174 – 1785Combined sources
Beta strandi183 – 1886Combined sources
Turni189 – 1924Combined sources
Beta strandi195 – 21218Combined sources
Turni213 – 2164Combined sources
Beta strandi231 – 2333Combined sources
Beta strandi238 – 2436Combined sources
Beta strandi245 – 2484Combined sources
Beta strandi257 – 2626Combined sources
Helixi266 – 2727Combined sources
Beta strandi278 – 2836Combined sources
Beta strandi285 – 2884Combined sources
Beta strandi290 – 2978Combined sources
Beta strandi302 – 3054Combined sources
Beta strandi307 – 3104Combined sources
Beta strandi312 – 32110Combined sources
Beta strandi326 – 3294Combined sources
Beta strandi331 – 3333Combined sources
Beta strandi336 – 3383Combined sources
Beta strandi340 – 3467Combined sources
Beta strandi351 – 3544Combined sources
Beta strandi356 – 3594Combined sources
Beta strandi364 – 3707Combined sources
Beta strandi375 – 3784Combined sources
Beta strandi380 – 3823Combined sources
Beta strandi388 – 3936Combined sources
Beta strandi398 – 4014Combined sources
Beta strandi403 – 4053Combined sources
Beta strandi411 – 4166Combined sources
Beta strandi419 – 4246Combined sources
Beta strandi426 – 4294Combined sources
Beta strandi431 – 4388Combined sources
Turni449 – 4513Combined sources
Beta strandi459 – 46911Combined sources
Beta strandi471 – 4777Combined sources
Beta strandi481 – 4877Combined sources
Beta strandi489 – 4913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NK6X-ray2.10A46-536[»]
4NK8X-ray2.29A46-536[»]
4OZYX-ray2.90A69-491[»]
4OZZX-ray2.90A69-492[»]
ProteinModelPortaliQ887Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati298 – 32023PbH1 1Add
BLAST
Repeati322 – 34524PbH1 2Add
BLAST
Repeati347 – 36923PbH1 3Add
BLAST
Repeati371 – 39323PbH1 4Add
BLAST
Repeati394 – 41623PbH1 5Add
BLAST

Sequence similaritiesi

Belongs to the D-mannuronate C5-epimerase family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108C0K. Bacteria.
ENOG41101XF. LUCA.
HOGENOMiHOG000270136.
KOiK01795.
OMAiHDRSHGL.
OrthoDBiEOG6D5FZ4.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR006633. Carb-bd_sugar_hydrolysis-dom.
IPR022441. Para_beta_helix_rpt-2.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SMARTiSM00722. CASH. 1 hit.
SM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR03804. para_beta_helix. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q887Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSHASNGRS RNWPHALLES ALLTSALLMA SSVALANAPA VPEAPKALVK
60 70 80 90 100
ELHQAKTYTI TSPPTGPLEM AKPVLPDLSG YTTEAALKKI ARNKPGKITV
110 120 130 140 150
ARMMEETGLK EFIGGDNKMA EWVVRQKGIP QAIMISDGYV NLQDLVKKVP
160 170 180 190 200
KQFLSEVSPG VYVARLPILV KETGIFEIDS KTKELRLSQE KGSFIVSEGK
210 220 230 240 250
MLITNTSVNA WSETRNGLAA YRTPDEFRPF VLTWGGSQTW IAKTKMASMG
260 270 280 290 300
YNQSKSYGVS ISQYTPNTAK VLKRGEPTGW IIDSEFADMW YGFYCYETRD
310 320 330 340 350
FVVKGNTYRD NIVYGIDPHD RSHGLIIAEN DVYGTKKKHG IIISREVDNS
360 370 380 390 400
FIFRNKSHNN KLSGVVLDRN SVGNIVAYNE IYQNHTDGIT LYESGNNLLW
410 420 430 440 450
GNRVIANRRH GIRVRNSVNI KLYENVAMAN GLMGVYGHIK DLNDTDRDIE
460 470 480 490 500
LDPFDAQVSL IMVGGELSSN GSGPLSIDSP LSVELYRVSM LMPTKEVGIS
510 520 530
LNGILGERQD EILDLLVRQK KAVLIDPVES QTELRE
Length:536
Mass (Da):59,486
Last modified:June 1, 2003 - v1
Checksum:iB17F41A67C6AA854
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO54763.1.
RefSeqiNP_791068.1. NC_004578.1.
WP_011103483.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO54763; AAO54763; PSPTO_1238.
GeneIDi1182874.
KEGGipst:PSPTO_1238.
PATRICi19993716. VBIPseSyr93040_1259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO54763.1.
RefSeqiNP_791068.1. NC_004578.1.
WP_011103483.1. NC_004578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NK6X-ray2.10A46-536[»]
4NK8X-ray2.29A46-536[»]
4OZYX-ray2.90A69-491[»]
4OZZX-ray2.90A69-492[»]
ProteinModelPortaliQ887Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_1238.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO54763; AAO54763; PSPTO_1238.
GeneIDi1182874.
KEGGipst:PSPTO_1238.
PATRICi19993716. VBIPseSyr93040_1259.

Phylogenomic databases

eggNOGiENOG4108C0K. Bacteria.
ENOG41101XF. LUCA.
HOGENOMiHOG000270136.
KOiK01795.
OMAiHDRSHGL.
OrthoDBiEOG6D5FZ4.

Enzyme and pathway databases

UniPathwayiUPA00286.
BioCyciPSYR223283:GJIX-1263-MONOMER.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR006633. Carb-bd_sugar_hydrolysis-dom.
IPR022441. Para_beta_helix_rpt-2.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SMARTiSM00722. CASH. 1 hit.
SM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR03804. para_beta_helix. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiALGG_PSESM
AccessioniPrimary (citable) accession number: Q887Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2003
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.