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Q887P8

- ALGD_PSESM

UniProt

Q887P8 - ALGD_PSESM

Protein

GDP-mannose 6-dehydrogenase

Gene

algD

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics By similarity.By similarity

    Catalytic activityi

    GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei10 – 101NAD bindingBy similarity
    Sitei11 – 111NAD bindingBy similarity
    Sitei30 – 301NAD bindingBy similarity
    Sitei35 – 351NAD bindingBy similarity
    Sitei86 – 861NAD bindingBy similarity
    Sitei161 – 1611NAD bindingBy similarity
    Active sitei268 – 2681By similarity
    Sitei271 – 2711NAD bindingBy similarity
    Sitei331 – 3311NAD bindingBy similarity

    GO - Molecular functioni

    1. GDP-mannose 6-dehydrogenase activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. alginic acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Alginate biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-1268-MONOMER.
    UniPathwayiUPA00286.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDP-mannose 6-dehydrogenase (EC:1.1.1.132)
    Short name:
    GMD
    Gene namesi
    Name:algD
    Ordered Locus Names:PSPTO_1243
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 438438GDP-mannose 6-dehydrogenasePRO_0000074070Add
    BLAST

    Expressioni

    Inductioni

    By oxidative compounds such as H2O2.

    Interactioni

    Protein-protein interaction databases

    STRINGi223283.PSPTO_1243.

    Structurei

    3D structure databases

    ProteinModelPortaliQ887P8.
    SMRiQ887P8. Positions 1-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.
    HOGENOMiHOG000153773.
    KOiK00066.
    OMAiGDLELDY.
    OrthoDBiEOG6ZSP7N.
    PhylomeDBiQ887P8.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR028358. GDPman_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500135. GDPman_DH. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q887P8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG    50
    LEELLQKGLA TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE 100
    SVCREIGYVL RDKNTRHTIV VRSTVLPGTV ANVVIPILED CSGKKAGVDF 150
    GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD KASGDVLQSL YEELDAPIIR 200
    KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE VMDVVCQDKA 250
    LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVDAPLL NSLMRSNTSQ 300
    VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS 350
    IFDSNVEYAR VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD 400
    ERFRSLANKT PEGKRVIDLV GFMTNATTED GRAEGICW 438
    Length:438
    Mass (Da):47,608
    Last modified:June 1, 2003 - v1
    Checksum:iB027F5AEF9F06E16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY095346 Genomic DNA. Translation: AAM23310.1.
    AE016853 Genomic DNA. Translation: AAO54768.1.
    RefSeqiNP_791073.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO54768; AAO54768; PSPTO_1243.
    GeneIDi1182879.
    KEGGipst:PSPTO_1243.
    PATRICi19993726. VBIPseSyr93040_1264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY095346 Genomic DNA. Translation: AAM23310.1 .
    AE016853 Genomic DNA. Translation: AAO54768.1 .
    RefSeqi NP_791073.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q887P8.
    SMRi Q887P8. Positions 1-438.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_1243.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO54768 ; AAO54768 ; PSPTO_1243 .
    GeneIDi 1182879.
    KEGGi pst:PSPTO_1243.
    PATRICi 19993726. VBIPseSyr93040_1264.

    Phylogenomic databases

    eggNOGi COG1004.
    HOGENOMi HOG000153773.
    KOi K00066.
    OMAi GDLELDY.
    OrthoDBi EOG6ZSP7N.
    PhylomeDBi Q887P8.

    Enzyme and pathway databases

    UniPathwayi UPA00286 .
    BioCyci PSYR223283:GJIX-1268-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR028358. GDPman_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500135. GDPman_DH. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the algD promoter, gene and protein from Pseudomonas syringae, Pseudomonas aeruginosa, and Azotobacter vinelandii."
      Keith R.C., Keith L.M., Bender C.L.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DC3000.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.
    3. "Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host and non-host plants."
      Keith R.C., Keith L.M., Hernandez-Guzman G., Uppalapati S.R., Bender C.L.
      Microbiology 149:1127-1138(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: EXPRESSION IN HOST.
      Strain: DC3000.

    Entry informationi

    Entry nameiALGD_PSESM
    AccessioniPrimary (citable) accession number: Q887P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3