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Q887P8 (ALGD_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-mannose 6-dehydrogenase
Short name=GMD
EC=1.1.1.132
Gene names
Name:algD
Ordered Locus Names:PSPTO_1243
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics By similarity.

Catalytic activity

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathway

Glycan biosynthesis; alginate biosynthesis.

Induction

By oxidative compounds such as H2O2.

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
   Biological processAlginate biosynthesis
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processalginic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionGDP-mannose 6-dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438GDP-mannose 6-dehydrogenase
PRO_0000074070

Sites

Active site2681 By similarity
Site101NAD binding By similarity
Site111NAD binding By similarity
Site301NAD binding By similarity
Site351NAD binding By similarity
Site861NAD binding By similarity
Site1611NAD binding By similarity
Site2711NAD binding By similarity
Site3311NAD binding By similarity

Sequences

Sequence LengthMass (Da)Tools
Q887P8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: B027F5AEF9F06E16

FASTA43847,608
        10         20         30         40         50         60 
MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG LEELLQKGLA 

        70         80         90        100        110        120 
TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE SVCREIGYVL RDKNTRHTIV 

       130        140        150        160        170        180 
VRSTVLPGTV ANVVIPILED CSGKKAGVDF GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD 

       190        200        210        220        230        240 
KASGDVLQSL YEELDAPIIR KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE 

       250        260        270        280        290        300 
VMDVVCQDKA LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVDAPLL NSLMRSNTSQ 

       310        320        330        340        350        360 
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS IFDSNVEYAR 

       370        380        390        400        410        420 
VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD ERFRSLANKT PEGKRVIDLV 

       430 
GFMTNATTED GRAEGICW

« Hide

References

« Hide 'large scale' references
[1]"Comparative analysis of the algD promoter, gene and protein from Pseudomonas syringae, Pseudomonas aeruginosa, and Azotobacter vinelandii."
Keith R.C., Keith L.M., Bender C.L.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DC3000.
[2]"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000."
Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. expand/collapse author list , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A.
Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DC3000.
[3]"Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host and non-host plants."
Keith R.C., Keith L.M., Hernandez-Guzman G., Uppalapati S.R., Bender C.L.
Microbiology 149:1127-1138(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPRESSION IN HOST.
Strain: DC3000.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY095346 Genomic DNA. Translation: AAM23310.1.
AE016853 Genomic DNA. Translation: AAO54768.1.
RefSeqNP_791073.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ887P8.
SMRQ887P8. Positions 1-438.
ModBaseSearch...

Protein-protein interaction databases

STRING223283.PSPTO_1243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO54768; AAO54768; PSPTO_1243.
GeneID1182879.
KEGGpst:PSPTO_1243.
PATRIC19993726. VBIPseSyr93040_1264.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1004.
HOGENOMHOG000153773.
KOK00066.
OMAVCREIGY.
ProtClustDBCLSK868056.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-1268-MONOMER.
UniPathwayUPA00286.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. Nucleotide_sugar_DH.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMsTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALGD_PSESM
AccessionPrimary (citable) accession number: Q887P8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents