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Protein

GDP-mannose 6-dehydrogenase

Gene

algD

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics (By similarity).By similarity

Catalytic activityi

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathway:ialginate biosynthesis

This protein is involved in the pathway alginate biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway alginate biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101NAD bindingBy similarity
Sitei11 – 111NAD bindingBy similarity
Sitei30 – 301NAD bindingBy similarity
Sitei35 – 351NAD bindingBy similarity
Sitei86 – 861NAD bindingBy similarity
Sitei161 – 1611NAD bindingBy similarity
Active sitei268 – 2681By similarity
Sitei271 – 2711NAD bindingBy similarity
Sitei331 – 3311NAD bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-1268-MONOMER.
UniPathwayiUPA00286.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose 6-dehydrogenase (EC:1.1.1.132)
Short name:
GMD
Gene namesi
Name:algD
Ordered Locus Names:PSPTO_1243
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438GDP-mannose 6-dehydrogenasePRO_0000074070Add
BLAST

Expressioni

Inductioni

By oxidative compounds such as H2O2.

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1243.

Structurei

3D structure databases

ProteinModelPortaliQ887P8.
SMRiQ887P8. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
KOiK00066.
OMAiELVYNPE.
OrthoDBiEOG6ZSP7N.
PhylomeDBiQ887P8.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q887P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG
60 70 80 90 100
LEELLQKGLA TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE
110 120 130 140 150
SVCREIGYVL RDKNTRHTIV VRSTVLPGTV ANVVIPILED CSGKKAGVDF
160 170 180 190 200
GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD KASGDVLQSL YEELDAPIIR
210 220 230 240 250
KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE VMDVVCQDKA
260 270 280 290 300
LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVDAPLL NSLMRSNTSQ
310 320 330 340 350
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS
360 370 380 390 400
IFDSNVEYAR VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD
410 420 430
ERFRSLANKT PEGKRVIDLV GFMTNATTED GRAEGICW
Length:438
Mass (Da):47,608
Last modified:June 1, 2003 - v1
Checksum:iB027F5AEF9F06E16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY095346 Genomic DNA. Translation: AAM23310.1.
AE016853 Genomic DNA. Translation: AAO54768.1.
RefSeqiNP_791073.1. NC_004578.1.
WP_005764140.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO54768; AAO54768; PSPTO_1243.
GeneIDi1182879.
KEGGipst:PSPTO_1243.
PATRICi19993726. VBIPseSyr93040_1264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY095346 Genomic DNA. Translation: AAM23310.1.
AE016853 Genomic DNA. Translation: AAO54768.1.
RefSeqiNP_791073.1. NC_004578.1.
WP_005764140.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ887P8.
SMRiQ887P8. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_1243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO54768; AAO54768; PSPTO_1243.
GeneIDi1182879.
KEGGipst:PSPTO_1243.
PATRICi19993726. VBIPseSyr93040_1264.

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
KOiK00066.
OMAiELVYNPE.
OrthoDBiEOG6ZSP7N.
PhylomeDBiQ887P8.

Enzyme and pathway databases

UniPathwayiUPA00286.
BioCyciPSYR223283:GJIX-1268-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative analysis of the algD promoter, gene and protein from Pseudomonas syringae, Pseudomonas aeruginosa, and Azotobacter vinelandii."
    Keith R.C., Keith L.M., Bender C.L.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DC3000.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.
  3. "Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host and non-host plants."
    Keith R.C., Keith L.M., Hernandez-Guzman G., Uppalapati S.R., Bender C.L.
    Microbiology 149:1127-1138(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION IN HOST.
    Strain: DC3000.

Entry informationi

Entry nameiALGD_PSESM
AccessioniPrimary (citable) accession number: Q887P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.