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Q887P8

- ALGD_PSESM

UniProt

Q887P8 - ALGD_PSESM

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Protein
GDP-mannose 6-dehydrogenase
Gene
algD, PSPTO_1243
Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics By similarity.

Catalytic activityi

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101NAD binding By similarity
Sitei11 – 111NAD binding By similarity
Sitei30 – 301NAD binding By similarity
Sitei35 – 351NAD binding By similarity
Sitei86 – 861NAD binding By similarity
Sitei161 – 1611NAD binding By similarity
Active sitei268 – 2681 By similarity
Sitei271 – 2711NAD binding By similarity
Sitei331 – 3311NAD binding By similarity

GO - Molecular functioni

  1. GDP-mannose 6-dehydrogenase activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. alginic acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-1268-MONOMER.
UniPathwayiUPA00286.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose 6-dehydrogenase (EC:1.1.1.132)
Short name:
GMD
Gene namesi
Name:algD
Ordered Locus Names:PSPTO_1243
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438GDP-mannose 6-dehydrogenase
PRO_0000074070Add
BLAST

Expressioni

Inductioni

By oxidative compounds such as H2O2.

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1243.

Structurei

3D structure databases

ProteinModelPortaliQ887P8.
SMRiQ887P8. Positions 1-438.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
KOiK00066.
OMAiGDLELDY.
OrthoDBiEOG6ZSP7N.
PhylomeDBiQ887P8.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q887P8-1 [UniParc]FASTAAdd to Basket

« Hide

MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG    50
LEELLQKGLA TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE 100
SVCREIGYVL RDKNTRHTIV VRSTVLPGTV ANVVIPILED CSGKKAGVDF 150
GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD KASGDVLQSL YEELDAPIIR 200
KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE VMDVVCQDKA 250
LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVDAPLL NSLMRSNTSQ 300
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS 350
IFDSNVEYAR VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD 400
ERFRSLANKT PEGKRVIDLV GFMTNATTED GRAEGICW 438
Length:438
Mass (Da):47,608
Last modified:June 1, 2003 - v1
Checksum:iB027F5AEF9F06E16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY095346 Genomic DNA. Translation: AAM23310.1.
AE016853 Genomic DNA. Translation: AAO54768.1.
RefSeqiNP_791073.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO54768; AAO54768; PSPTO_1243.
GeneIDi1182879.
KEGGipst:PSPTO_1243.
PATRICi19993726. VBIPseSyr93040_1264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY095346 Genomic DNA. Translation: AAM23310.1 .
AE016853 Genomic DNA. Translation: AAO54768.1 .
RefSeqi NP_791073.1. NC_004578.1.

3D structure databases

ProteinModelPortali Q887P8.
SMRi Q887P8. Positions 1-438.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223283.PSPTO_1243.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO54768 ; AAO54768 ; PSPTO_1243 .
GeneIDi 1182879.
KEGGi pst:PSPTO_1243.
PATRICi 19993726. VBIPseSyr93040_1264.

Phylogenomic databases

eggNOGi COG1004.
HOGENOMi HOG000153773.
KOi K00066.
OMAi GDLELDY.
OrthoDBi EOG6ZSP7N.
PhylomeDBi Q887P8.

Enzyme and pathway databases

UniPathwayi UPA00286 .
BioCyci PSYR223283:GJIX-1268-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view ]
PANTHERi PTHR11374. PTHR11374. 1 hit.
Pfami PF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative analysis of the algD promoter, gene and protein from Pseudomonas syringae, Pseudomonas aeruginosa, and Azotobacter vinelandii."
    Keith R.C., Keith L.M., Bender C.L.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DC3000.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.
  3. "Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host and non-host plants."
    Keith R.C., Keith L.M., Hernandez-Guzman G., Uppalapati S.R., Bender C.L.
    Microbiology 149:1127-1138(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION IN HOST.
    Strain: DC3000.

Entry informationi

Entry nameiALGD_PSESM
AccessioniPrimary (citable) accession number: Q887P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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