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Q887B0 (TGT_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:PSPTO_1413
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168
PRO_0000135508

Sites

Active site891Nucleophile By similarity
Metal binding3001Zinc By similarity
Metal binding3021Zinc By similarity
Metal binding3051Zinc By similarity
Metal binding3311Zinc By similarity
Binding site901Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q887B0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7D056DB41B98E3A0

FASTA37140,978
        10         20         30         40         50         60 
MSFELLATDG KARRGRLTFP RGVVETPAFM PVGTYGTVKG MLPRDIEATG AQMILGNTFH 

        70         80         90        100        110        120 
LWLRPGTEVI KGHGDLHDFM QWKGPILTDS GGFQVFSLGA MRKIKEEGVT FASPVDGAKV 

       130        140        150        160        170        180 
FMGPEESMQV QRDLGSDVVM IFDECTPYPA DEDVARVSME LSLRWAKRSK VAHGENTAAL 

       190        200        210        220        230        240 
FGIVQGGMHE NLRKRSLEGL DEIGFDGLAI GGLSVGEPKH EMIKVLDYLP GLMPADKPRY 

       250        260        270        280        290        300 
LMGVGKPEDL VEGVRRGVDM FDCVMPTRNA RNGHLFIDTG VLKIRNAFHR HDNTPLDPTC 

       310        320        330        340        350        360 
DCYTCKNFSR AYLHHLDKCG EMLGSMLNTI HNLRHYQLLM AGLREAIQQG TLAAFVDAFY 

       370 
AKRGLPTPPL G

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016853 Genomic DNA. Translation: AAO54934.1.
RefSeqNP_791239.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ887B0.
SMRQ887B0. Positions 1-362.
ModBaseSearch...

Protein-protein interaction databases

STRING223283.PSPTO_1413.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO54934; AAO54934; PSPTO_1413.
GeneID1183050.
KEGGpst:PSPTO_1413.
PATRIC19994066. VBIPseSyr93040_1433.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-1440-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_PSESM
AccessionPrimary (citable) accession number: Q887B0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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