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Q886W6 (PUR4_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:PSPTO_1459
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12981298Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100415

Regions

Domain1045 – 1298254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11381Nucleophile By similarity
Active site12631 By similarity
Active site12651 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q886W6 [UniParc].

Last modified June 20, 2003. Version 1.
Checksum: 021B429EC6402AB6

FASTA1,298140,647
        10         20         30         40         50         60 
MLILRGAPAL SAFRHSKLLE QLKQKVSAVS GLYAEFAHFA DVNDVLTGEE QQVLDRLLKY 

        70         80         90        100        110        120 
GPSVPVQEPA GRLFLVLPRF GTISPWSSKA SDIARNCGLT KIQRIERGIA FYVEGQFSDA 

       130        140        150        160        170        180 
DAQAIADSLH DRMTQLVLGD HAQAAGLFSH AEPKPLTAVD ILGGGRAALE KANVELGLAL 

       190        200        210        220        230        240 
AEDEIDYLIT SFNGLGRNPH DIELMMFAQA NSEHCRHKIF NASWDIDGQS QEKSLFGMIK 

       250        260        270        280        290        300 
NTYEMHSEGV LSAYKDNASV IVGSVAGRFF PDPDTRQYGA VQEPVHILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFPGAATGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFVQPWEVP YGKPERIVTA 

       370        380        390        400        410        420 
LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQSITTPHGD EVRGYHKPIM LAGGMGNIRE 

       430        440        450        460        470        480 
DHVQKAEITV GSKLIVLGGP AMLIGLGGGA ASSMATGTSS ADLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DRNPISFIHD VGAGGLSNAF PELVNDGDRG GRFELRNVPN DEPGMAPLEI 

       550        560        570        580        590        600 
WSNESQERYV LAVGVADYER FKAICERERC PFAVVGEATA EPQLTVTDSH FGNSPVDMPL 

       610        620        630        640        650        660 
EVLLGKAPRM HRSVAREAEI GDDFDPSTLD IEESVQRVLR HPAVASKSFL ITIGDRSITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADCAV TATSFDVNTG EAMAMGERTP LALLDAPASG RMAIGETLTN 

       730        740        750        760        770        780 
IAASRIEKLS DIKLSANWMS AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMK 

       790        800        810        820        830        840 
TRWSDEGTEK SVTSPLSLIV TGFAPVVDIR KTLTPELRMD KGITDLILID LGRGQNRMGA 

       850        860        870        880        890        900 
SILAQTHGKL GRVAPDVDDA EDLKAFFAVI QGLNSDGHIL SYHDRSDGGL LVSTLEMAFA 

       910        920        930        940        950        960 
GHCGLNLHLD GLADNVSELS AILFNEELGA VIQVRQDATP LVLAQFSAAG LEDCVAVIGQ 

       970        980        990       1000       1010       1020 
PINNDEVSIS FLGEPVFSGQ RRLLQRQWAE TSYQIQRLRD NAECADQEFD ALLEEDNPGL 

      1030       1040       1050       1060       1070       1080 
TVKLGFDVNE DIAAPYIKTG VRPQVAVLRE QGVNGQVEMA AAFDRAGFNA IDVHMSDILA 

      1090       1100       1110       1120       1130       1140 
GRVDLNDFKG MVACGGFSYG DVLGAGEGWA KSALFNSRAR DAFQGFFERS DSFTLGVCNG 

      1150       1160       1170       1180       1190       1200 
CQMLSNLHEL IPGSEFWPHF VRNRSEQFEA RVAMVQVQES ASIFLQGMAG SRMPIAIAHG 

      1210       1220       1230       1240       1250       1260 
EGHAEFRNDD ALLEADVSGT VALRFVDNHG KVTESYPANP NGSPRGIGGM TTLDGRVTIM 

      1270       1280       1290 
MPHPERVFRA VQNSWRPEDW NEDAAWMRMF RNARAWVN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016853 Genomic DNA. Translation: AAO54980.1.
RefSeqNP_791285.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ886W6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1183096.
GenomeReviewsGene locus PSPTO_1459 in contig AE016853_GR.
KEGGpst:PSPTO_1459.
NMPDRfig|223283.1.peg.1423.
PATRIC19994162. VBIPseSyr93040_1481.
TIGRPSPTO_1459.

Phylogenomic databases

HOGENOMHBG335309.
OMAVKAVGME.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPSYR223283:PSPTO_1459-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_PSESM
AccessionPrimary (citable) accession number: Q886W6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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