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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  • [protein-PII] uridylyltransferase activity Source: JCVI
  • hydrolase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-1559-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PSPTO_1532
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927561 – 898Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST898

Proteomic databases

PRIDEiQ886P5

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1532

Structurei

3D structure databases

ProteinModelPortaliQ886P5
SMRiQ886P5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini460 – 582HDPROSITE-ProRule annotationAdd BLAST123
Domaini705 – 788ACT 1UniRule annotationAdd BLAST84
Domaini815 – 891ACT 2UniRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 341UridylyltransferaseAdd BLAST341
Regioni342 – 704Uridylyl-removingAdd BLAST363

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO
PhylomeDBiQ886P5

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

Q886P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQVDPDLFD RGQFQAELAL KASPIAAFKK AIRRARDVLD NRFRSGRDIR
60 70 80 90 100
RLIEDRAWFV DNILQQAWDQ FEWSEDADIA LLAVGGYGRG ELHPYSDIDL
110 120 130 140 150
LILLESDDHE VFREPIERFL TLLWDIGLEV GQSVRSVDEC AQEGRADLTV
160 170 180 190 200
ITNLMESRTI AGPEHLRQRM LEVTSTQHMW PSKEFFLAKH AEQKKRHHKY
210 220 230 240 250
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLHAL AGEGFLLGSE
260 270 280 290 300
NALLASSQEF LWKVRYALHM LAGRSEDRLL FDYQVRIAGL LGYEDNDAKL
310 320 330 340 350
AIERFMQKYY RVVMSIAELS DLIIQHFEEV ILSDDDGTPQ PINSRFQLHD
360 370 380 390 400
GYIEATNPNV FRRTPFAMLE IFVLMAQHPE IKGVRADTIR LLREHRHLIN
410 420 430 440 450
DDFRNDIRNT SLFIELFKCE IGIHRNLRRM NRYGILGLYL PEFGHIVGQM
460 470 480 490 500
QHDLFHIYTV DAHTLNLIKH LRKLQYTEVS EKFPLASKIM ARLPKPELIY
510 520 530 540 550
LAGLYHDIGK GRGGDHSELG AVDAQAFGTR HHLPAWDNRL IVWLVSNHLV
560 570 580 590 600
MSTTAQRKDL SDPQVIHDFA QFVGDEVHLD YLYVLTVADI NATNPTLWNS
610 620 630 640 650
WRASLLRQLY TETKRALRRG LENPVDREEQ IRRTQTAALD ILVRSGTDPD
660 670 680 690 700
DVEQLWSALG DDYFLRHTAG DVAWHSDAIL QQPADGGPLV LIKETTQREF
710 720 730 740 750
EGGTQIFIYA PDQHDFFAVT VAAMDQLNLN IHDARIITSS SQFTLDTYIV
760 770 780 790 800
LDHEGGSIGN NPERIQDIRD GLTEALRNPD DYPTIIKRRV PRQLKHFAFA
810 820 830 840 850
PQVTIHNDAQ RPVTVLELLA PDRPGLLARI GKIFLEFDLS LQNAKIATLG
860 870 880 890
ERVEDVFFIT DANNQPLSDP QLCSQLQEAI VKQLSVNSEP GGDLRISI
Length:898
Mass (Da):102,738
Last modified:June 1, 2003 - v1
Checksum:i09151491E148C501
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO55052.1
RefSeqiNP_791357.1, NC_004578.1
WP_007245412.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO55052; AAO55052; PSPTO_1532
GeneIDi1183169
KEGGipst:PSPTO_1532
PATRICifig|223283.9.peg.1556

Similar proteinsi

Entry informationi

Entry nameiGLND_PSESM
AccessioniPrimary (citable) accession number: Q886P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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