Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q886P5

- GLND_PSESM

UniProt

Q886P5 - GLND_PSESM

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-1559-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:PSPTO_1532
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 898898Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192756Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi223283.PSPTO_1532.

    Structurei

    3D structure databases

    ProteinModelPortaliQ886P5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini463 – 567105HDUniRule annotationAdd
    BLAST
    Domaini705 – 78884ACT 1UniRule annotationAdd
    BLAST
    Domaini815 – 89177ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 341341UridylyltransferaseAdd
    BLAST
    Regioni342 – 704363Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.
    PhylomeDBiQ886P5.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q886P5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQVDPDLFD RGQFQAELAL KASPIAAFKK AIRRARDVLD NRFRSGRDIR    50
    RLIEDRAWFV DNILQQAWDQ FEWSEDADIA LLAVGGYGRG ELHPYSDIDL 100
    LILLESDDHE VFREPIERFL TLLWDIGLEV GQSVRSVDEC AQEGRADLTV 150
    ITNLMESRTI AGPEHLRQRM LEVTSTQHMW PSKEFFLAKH AEQKKRHHKY 200
    NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLHAL AGEGFLLGSE 250
    NALLASSQEF LWKVRYALHM LAGRSEDRLL FDYQVRIAGL LGYEDNDAKL 300
    AIERFMQKYY RVVMSIAELS DLIIQHFEEV ILSDDDGTPQ PINSRFQLHD 350
    GYIEATNPNV FRRTPFAMLE IFVLMAQHPE IKGVRADTIR LLREHRHLIN 400
    DDFRNDIRNT SLFIELFKCE IGIHRNLRRM NRYGILGLYL PEFGHIVGQM 450
    QHDLFHIYTV DAHTLNLIKH LRKLQYTEVS EKFPLASKIM ARLPKPELIY 500
    LAGLYHDIGK GRGGDHSELG AVDAQAFGTR HHLPAWDNRL IVWLVSNHLV 550
    MSTTAQRKDL SDPQVIHDFA QFVGDEVHLD YLYVLTVADI NATNPTLWNS 600
    WRASLLRQLY TETKRALRRG LENPVDREEQ IRRTQTAALD ILVRSGTDPD 650
    DVEQLWSALG DDYFLRHTAG DVAWHSDAIL QQPADGGPLV LIKETTQREF 700
    EGGTQIFIYA PDQHDFFAVT VAAMDQLNLN IHDARIITSS SQFTLDTYIV 750
    LDHEGGSIGN NPERIQDIRD GLTEALRNPD DYPTIIKRRV PRQLKHFAFA 800
    PQVTIHNDAQ RPVTVLELLA PDRPGLLARI GKIFLEFDLS LQNAKIATLG 850
    ERVEDVFFIT DANNQPLSDP QLCSQLQEAI VKQLSVNSEP GGDLRISI 898
    Length:898
    Mass (Da):102,738
    Last modified:June 1, 2003 - v1
    Checksum:i09151491E148C501
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO55052.1.
    RefSeqiNP_791357.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO55052; AAO55052; PSPTO_1532.
    GeneIDi1183169.
    KEGGipst:PSPTO_1532.
    PATRICi19994312. VBIPseSyr93040_1556.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO55052.1 .
    RefSeqi NP_791357.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q886P5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_1532.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO55052 ; AAO55052 ; PSPTO_1532 .
    GeneIDi 1183169.
    KEGGi pst:PSPTO_1532.
    PATRICi 19994312. VBIPseSyr93040_1556.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.
    PhylomeDBi Q886P5.

    Enzyme and pathway databases

    BioCyci PSYR223283:GJIX-1559-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.

    Entry informationi

    Entry nameiGLND_PSESM
    AccessioniPrimary (citable) accession number: Q886P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3