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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-1575-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:PSPTO_1547
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901791 – 380Lipid-A-disaccharide synthaseAdd BLAST380

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1547

Structurei

3D structure databases

ProteinModelPortaliQ886N0
SMRiQ886N0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018004
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW
PhylomeDBiQ886N0

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

Q886N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPLRIALV AGEASGDILG SGLMRALKAR HPDVRFIGVG GPLMEAEGMQ
60 70 80 90 100
SYFPMERLSV MGLVEVLGRL RELLARRKLL VQTLIDEKPD VFIGIDAPDF
110 120 130 140 150
TLNIELQLRR AGIKTVHYVS PSVWAWRQKR VLKIREGCDL MLTLLPFEAR
160 170 180 190 200
FYEEKGVPVR FVGHPLADTI PLESDRGATR AELGLSVDGP VVALMPGSRG
210 220 230 240 250
GEVGRLGALF FDAAERLLVE RPGLRFVLPC ASPQRRAQVE QLLQGRDLPI
260 270 280 290 300
TLLDGRSHVA LAACDAVLIA SGTATLEALL YKRPMVVAYR MAPLTFWVLK
310 320 330 340 350
RLVKSPYVSL PNLLAQRLLV PELLQDDATP EALARTLLPL IEDGHAQTEG
360 370 380
FDAIHRILRR DASNQAADAV LSLLGLPLSL
Length:380
Mass (Da):41,686
Last modified:June 1, 2003 - v1
Checksum:iD78706BD0A6E3FF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO55067.1
RefSeqiNP_791372.1, NC_004578.1
WP_011103595.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO55067; AAO55067; PSPTO_1547
GeneIDi1183184
KEGGipst:PSPTO_1547
PATRICifig|223283.9.peg.1573

Similar proteinsi

Entry informationi

Entry nameiLPXB_PSESM
AccessioniPrimary (citable) accession number: Q886N0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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