Q885V0 (FUMC_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC-1
Ordered Locus Names:	PSPTO_1731

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161302
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q885V0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6B12FFF27257FFD2	FASTA	464	49,077
	10 20 30 40 50 60 MSRTETDSIG PIEVPEDAYW GAQTQRSLIN FAIGDQRMPL AVLHALTLIK KAAARVNDRN 70 80 90 100 110 120 GDLPADIARL IEQAADEVLD GQHDAQFPLV VWQTGSGTQS NMNVNEVIAG RANELAGQGR 130 140 150 160 170 180 GGKSPVHPND HVNRSQSSND CFPTAMHIAT AQAVKEQLLP AIAELSSGLA EQAARHMKLV 190 200 210 220 230 240 KTGRTHMMDA TPITFGQELS GFVAQLDYAE KAIRAALPAV YELAQGGTAV GTGLNAPKGF 250 260 270 280 290 300 AEAIAAELAA LSGLPFVTAP NKFAALAGHE PLAALSGALK TLAGTLMKIA NDLRLLGSGP 310 320 330 340 350 360 RAGLAEVRLP ANEPGSSIMP GKVNPTQCEA LSMLACQVMG NDVTIGFAAS QGHLQLNVYK 370 380 390 400 410 420 PVIIHNVLQS IRLLADGCSN FNEHCVAGME PDAEKMAEHL ERGLMLVTAL NPHIGYDKSA 430 440 450 460 HIAKKAYTEG LTLREAALAL GYLTDEEFDA WVRPDKMLEA GSNG

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO55251.1.
RefSeq	NP_791556.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q885V0.
SMR	Q885V0. Positions 3-458.
ModBase	Search...
2D gel databases
World-2DPAGE	0008:Q885V0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1183368.
GenomeReviews	Gene locus PSPTO_1731 in contig AE016853_GR.
KEGG	pst:PSPTO_1731.
NMPDR	fig|223283.1.peg.1694.
PATRIC	19994714. VBIPseSyr93040_1758.
TIGR	PSPTO_1731.
Phylogenomic databases
HOGENOM	HBG284369.
OMA	AGHEPLT.
ProtClustDB	PRK12425.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_1731-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_PSESM

Accession

Primary (citable) accession number: Q885V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program