Reviewed, UniProtKB/Swiss-Prot Q885J7 (ASTD_PSESM)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	PSPTO_1835

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_0000262419
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q885J7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9FD45FF64E7B8486	FASTA	488	51,252
	10 20 30 40 50 60 MSTLYIAGAW QAGQGELFHS LNPVTLHVLW SGQGATAEQV DHAVQAARQA FPAWALLSLD 70 80 90 100 110 120 QRIAVLDAFA VSLKAHADEL ANCIGEETGK PLWESATEVT SMVNKIAISV QSYRERTGEK 130 140 150 160 170 180 SAPLGDATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNTVLFKP SELTPKVAEL 190 200 210 220 230 240 TVKCWIEAGL PAGVLNLLQG GRETGIALAA NPGIDGLFFT GSSRTGNALH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LVVDQVQDID AAVYTIIQSA FISAGQRCTC ARRLLVPEGD WGDALLARLV 310 320 330 340 350 360 AVSATLDVGA FDQQPAPFMG SVISLEAARA LLDAQRHLLA NGAVTLLEMR QPQPGAALLT 370 380 390 400 410 420 PGIVDVSAVA NRPDVELFGP LLQVVRYAGF DAAIAEANAT QYGLAAGLLS DSEARYQQFW 430 440 450 460 470 480 LQSRAGIVNW NKQLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL ETGSLTLPAT LTPGVRLS

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO55354.1.
RefSeq	NP_791659.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1183476.
GenomeReviews	Gene locus PSPTO_1835 in contig AE016853_GR.
KEGG	pst:PSPTO_1835.
NMPDR	fig|223283.1.peg.1797.
TIGR	PSPTO_1835.
Phylogenomic databases
HOGENOM	Q885J7.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_1835-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSESM

Accession

Primary (citable) accession number: Q885J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)