Q885J4 (ASTE_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	PSPTO_1838

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 3 › Pseudomonas syringae pv. tomato

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP-Rule MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP-Rule MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP-Rule MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP arginine catabolic process to succinate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Succinylglutamate desuccinylase HAMAP-Rule MF_00767		PRO_0000174645
Sites
	Active site	215	1	Potential
	Metal binding	59	1	Zinc By similarity
	Metal binding	62	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q885J4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E43CFD5829B97F3C	FASTA	335	37,375
	10 20 30 40 50 60 MLALGKLLEL TLAGREPAEK TQLTVEGVRM RWLAEGALEV RPPQARDNGT DLLLSAGIHG 70 80 90 100 110 120 NETAPIELLD ELIRSIARGD LKPRARILFL FGNPAAMRLG ARYVEQDVNR LFNGRHEQSG 130 140 150 160 170 180 GAEALRACEL ERLAASFFSL PDRYRLHYDL HTAIRGSKIE QFALYPWKEG RQHSRFELAR 190 200 210 220 230 240 LRAAGISAVL LQNKPSIVFS AYTYEQLGAE AFTLELGKAR PFGQNRHVNL APLRLRLEQI 250 260 270 280 290 300 IEGSEPQPDE RLEGLQLFSV AREVIKRSDA FTFNLADDVE NFSELEKGYV LAEDVSDSRW 310 320 330 VVKEEGARII FPNPKVKNGL RAGIVIVPAD ADGLG

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO55357.1.
RefSeq	NP_791662.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q885J4.
ModBase	Search...
Protein-protein interaction databases
STRING	223283.PSPTO_1838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO55357; AAO55357; PSPTO_1838.
GeneID	1183479.
KEGG	pst:PSPTO_1838.
PATRIC	19994942. VBIPseSyr93040_1868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2988.
HOGENOM	HOG000280300.
KO	K05526.
OMA	TAPIEIC.
ProtClustDB	PRK05324.
Enzyme and pathway databases
BioCyc	PSYR223283:GJIX-1873-MONOMER.
UniPathway	UPA00185; UER00283.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE.
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_PSESM

Accession

Primary (citable) accession number: Q885J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program