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Q884I1 (QUEF_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:PSPTO_2112
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: HAMAP

preQ1 synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817
PRO_0000163049

Sequences

Sequence LengthMass (Da)Tools
Q884I1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 693A2CCACBE7D9D2

FASTA27630,751
        10         20         30         40         50         60 
MHPAAEHSPL GKSSEYIATY TPSLLFPIPR AAKWAELGLT AQTLPYKGVD FWNCYELSWL 

        70         80         90        100        110        120 
LPSGKPVVAI AEFSIPADSP NIIESKSFKL YLNSLNQTAF DGAARVQATL EKDLSAAAGK 

       130        140        150        160        170        180 
LVGVRIRNLA EIEEEGVAVL PGVCIDELDI TVSSYDQPQP ELLRCDDSQV IEEAVHSHLL 

       190        200        210        220        230        240 
KSNCPVTSQP DWGSVVVEYR GAALDHASLL AYIVSFRQHS DFHEQCVERI FLDLQRLLKP 

       250        260        270 
EKLTVYARYV RRGGLDINPY RSTEVLDVDN RRLARQ

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016853 Genomic DNA. Translation: AAO55629.1.
RefSeqNP_791934.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ884I1.
SMRQ884I1. Positions 16-276.
ModBaseSearch...

Protein-protein interaction databases

STRING223283.PSPTO_2112.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO55629; AAO55629; PSPTO_2112.
GeneID1183759.
KEGGpst:PSPTO_2112.
PATRIC19995502. VBIPseSyr93040_2143.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHOG000273755.
KOK06879.
OMAERIFCDL.
ProtClustDBPRK11792.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-2153-MONOMER.
UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR016428. CN_OxRdtase_NADPH-dep_YqcD.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_PSESM
AccessionPrimary (citable) accession number: Q884I1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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