Reviewed, UniProtKB/Swiss-Prot Q88434 (L_SV5)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Simian virus 5 (strain W3) (SV5)

Taxonomic identifier

11208 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Canis familiaris (Dog) [TaxID: 9615]

Protein attributes

Sequence length	2255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxoviruses L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2255	2255	Large structural protein		PRO_0000142742
Regions
	Domain	656 – 840	185	RdRp catalytic
	Nucleotide binding	1805 – 1814	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q88434-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 63EE2994D68DDE38	FASTA	2,255	255,926
	10 20 30 40 50 60 MAGSREILLP EVHLNSPIVK HKLYYYILLG NLPNEIDLDD LGPLHNQNWN QIAHEESNLA 70 80 90 100 110 120 QRLVNVRNFL ITHIPDLRKG HWQEYVNVIL WPRILPLIPD FKINDQLPLL KNWDKLVKES 130 140 150 160 170 180 CSVINAGTSQ CIQNLSYGLT GRGNLFTRSR ELSGDRRDID LKTVVAAWHD SDWKRISDFW 190 200 210 220 230 240 IMIKFQMRQL IVRQTDHNDS DLITYIENRE GIIIITPELV ALFNTENHTL TYMTFEIVLM 250 260 270 280 290 300 VSDMYEGRHN ILSLCTVSTY LNPLKKRITY LLSLVDNLAF QIGDAVYNII ALLESFVYAQ 310 320 330 340 350 360 LQMSDPIPEL RGQFHAFVCS EILDALRGTN SFTQDELRTV TTNLISPFQD LTPDLTAELL 370 380 390 400 410 420 CIMRLWGHPM LTASQAAGKV RESMCAGKVL DFPTIMKTLA FFHTILINGY RRKHHGVWPP 430 440 450 460 470 480 LNLPGNASKG LTELMNDNTE ISYEFTLKHW KEVSLIKFKK CFDADAGEEL SIFMKDKAIS 490 500 510 520 530 540 APKQDWMSVF RRSLIKQRHQ HHQVPLPNPF NRRLLLNFLG DDKFDPNVEL QYVTSGEYLH 550 560 570 580 590 600 DDTFCASYSL KEKEIKPDGR IFAKLTKRMR SCQVIAESLL ANHAGKLMKE NGVVMNQLSL 610 620 630 640 650 660 TKSLLTMSQI GIISEKARKS TRDNINQPGF QNIQRNKSHH SKQVNQRDPS DDFELAASFL 670 680 690 700 710 720 TTDLKKYCLQ WRYQTIIPFA QSLNRMYGYP HLFEWIHLRL MRSTLYVGDP FNPPADTSQF 730 740 750 760 770 780 DLDKVINGDI FIVSPRGGIE GLCQKAWTMI SIAVIILSAT ESGTRVMSMV QGDNQAIAVT 790 800 810 820 830 840 TRVPRSLPTL EKKTIAFRSC NLFFERLKCN NFGLGHHLKE QETIISSHFF VYSKRIFYQG 850 860 870 880 890 900 RILTQALKNA SKLCLTADVL GECTQSSCSN LATTVMRLTE NGVEKDICFY LNIYMTIKQL 910 920 930 940 950 960 SYDIIFPQVS IPGDQITLEY INNPHLVSRL ALLPSQLGGL NYLSCSRLFN RNIGDPVVSA 970 980 990 1000 1010 1020 VADLKRLIKS GCMDYWILYN LLGRKPGNGS WATLAADPYS INIEYQYPPT TALKRHTQQA 1030 1040 1050 1060 1070 1080 LMELSTNPML RGIFSDNAQA EENNLARFLL DREVIFPRVA HIIIEQTSVG RRKQIQGYLD 1090 1100 1110 1120 1130 1140 STRSIMRKSL EIKPLSNRKL NEILDYNINY LAYNLALLKN AIEPPTYLKA MTLETCSIDI 1150 1160 1170 1180 1190 1200 ARNLRKLSWA PLLGGRNLEG LETPDPIEIT AGALIVGSGY CEQCAAGDNR FTWFFLPSGI 1210 1220 1230 1240 1250 1260 EIGGDPRDNP PIRVPYIGSR TDERRVASMA YIRGASSSLK AVLRLAGVYI WAFGDTLENW 1270 1280 1290 1300 1310 1320 IDALDLSHTR VNITLEQLQS LTPLPTSANL THRLDDGTTT LKFTPASSYT FSSFTHISND 1330 1340 1350 1360 1370 1380 EQYLTINDKT ADSNIIYQQL MITGLGILET WNNPPINRTF EESTLHLHTG ASCCVRPVDS 1390 1400 1410 1420 1430 1440 CILSEALTVK PHITVPYSNK FVFDEDPLSE YETAKLESLS FQAQLGNIDA VDMTGKLTLL 1450 1460 1470 1480 1490 1500 SQFTARQIIN AITGLDESVS LTNDAIVASD YVSNWISECM YTKLDELFMY CGWELLLELS 1510 1520 1530 1540 1550 1560 YQMYYLRVVG WSNIVDYSYM ILRRIPGAAL NNLASTLSHP KLFRRAINLD IVAPLNAPHF 1570 1580 1590 1600 1610 1620 ASLDYIKMSV DAILWGCKRV INVLSNGGDL ELVVTSEDSL ILSDRSMNLI ARKLTLLSLI 1630 1640 1650 1660 1670 1680 HHNGLELPKI KGFSPDEKCF ALTEFLRKVV NSGLSSIENL SNFMYNVENP RLAAFASNNY 1690 1700 1710 1720 1730 1740 YLTRKLLNSI RDTESGQVAV TSYYESLEYI DSLKLTPHVP GTSCIEDDSL CTNDYIIWII 1750 1760 1770 1780 1790 1800 ESNANLEKYP IPNSPEDDSN FHNFKLNAPS HHTLRPLGLS STAWYKGISC CRYLERLKLP 1810 1820 1830 1840 1850 1860 QGDHLYIAEG SGASMTIIEY LFPGRKIYYN SLFSSGDNPP QRNYAPMPTQ FIESVPYKLW 1870 1880 1890 1900 1910 1920 QAHTDQYPEI FEDFIPLWNG NAAMTDIGMT ACVEFIINRV GPRTCSLVHV DLESSASLNQ 1930 1940 1950 1960 1970 1980 QCLSKPIINA IITATTVLCP HGVLILKYSW LPFTRFSTLI TFLWCYFERI TVLRSTYSDP 1990 2000 2010 2020 2030 2040 ANHEVYLICI LANNFAFQTV SQATGMAMTL TDQGFTLISP ERINQYWDGH LKQERIVAEA 2050 2060 2070 2080 2090 2100 IDKVVLGENA LFNSSDNELI LKCGGTPNAR NLIDIEPVAT FIEFEQLICT MLTTHLKEII 2110 2120 2130 2140 2150 2160 DITRSGTQDY ESLLLTPYNL GLLGKISTIV RLLTERILNH TIRNWLILPP SLRMIVKQDL 2170 2180 2190 2200 2210 2220 EFGIFRITSI LNSDRFLKLS PNRKYLIAQL TAGYIRKLIE GDCNIDLTRP IQKQIWKALG 2230 2240 2250 CVVYCHDPMD QRESTEFIDI NINEEIDRGI DGEEI

References

[1]	"Molecular cloning of the NP and L genes of simian virus 5: identification of highly conserved domains in paramyxovirus NP and L proteins." Parks G.D., Ward C.D., Lamb R.A. Virus Res. 22:259-279(1992) [PubMed: 1320792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81721 Genomic RNA. Translation: AAA47879.1. AF052755 Genomic RNA. Translation: AAC95518.1.
RefSeq	YP_138518.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3160798.
Family and domain databases
InterPro	IPR016269. RNA-dir_RNA_pol_paramyxovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SV5

Accession

Primary (citable) accession number: Q88434

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)