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Protein

Glutaminase

Gene

glsA

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611SubstrateUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Binding sitei155 – 1551SubstrateUniRule annotation
Binding sitei162 – 1621SubstrateUniRule annotation
Binding sitei186 – 1861SubstrateUniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation
Binding sitei256 – 2561Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-2533-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GlutaminaseUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:glsAUniRule annotation
Ordered Locus Names:PSPTO_2488
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302GlutaminasePRO_0000110617Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_2488.

Structurei

3D structure databases

ProteinModelPortaliQ882Y4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.
PhylomeDBiQ882Y4.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q882Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHDLLNEILD EVRPLLGQGK VADYIPALGG VRPDQLGIAV YGNDGQVFSA
60 70 80 90 100
GDAETLFSIQ SISKVFSLVQ AIGHSGEDIW QRLGHEPSGQ PFNSLVQLEF
110 120 130 140 150
EQGRPRNPFI NAGALVICDI NQARFAAPSL SMRDFVRRLC GNRSITSDSK
160 170 180 190 200
VAESEYQHRS RNAAAAYLMK SFDNFHGDVE DVLRSYFHHC ALSMNCIDLA
210 220 230 240 250
KAFGFLANQG FCAHSGVQIL TARQATQVNS IMATSGLYDE AGNFAYRVGL
260 270 280 290 300
PGKSGVGGGI VAIVPERASV CVWSPALNAA GNSLVGMAAL EKLSARVDWS

VF
Length:302
Mass (Da):32,524
Last modified:June 1, 2003 - v1
Checksum:iCF6DC4B0EB2E50DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO55995.1.
RefSeqiNP_792300.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO55995; AAO55995; PSPTO_2488.
GeneIDi1184140.
KEGGipst:PSPTO_2488.
PATRICi19996283. VBIPseSyr93040_2528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO55995.1.
RefSeqiNP_792300.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ882Y4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_2488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO55995; AAO55995; PSPTO_2488.
GeneIDi1184140.
KEGGipst:PSPTO_2488.
PATRICi19996283. VBIPseSyr93040_2528.

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.
PhylomeDBiQ882Y4.

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-2533-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiGLSA_PSESM
AccessioniPrimary (citable) accession number: Q882Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.