Reviewed, UniProtKB/Swiss-Prot Q88261 (HN_SENDO)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
      Short name=HN protein
      Short name=Protein HANA
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Sendai virus (strain Ohita) (SeV) [Complete proteome]

Taxonomic identifier

302272 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subunit structure	Homotetramer; composed of disulfide-linked homodimers. Interacts with F protein trimer By similarity.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential. Note: Folded in the endoplasmic reticulum By similarity.
Post-translational modification	N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides By similarity.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Hemagglutinin-neuraminidase		PRO_0000142639
Regions
	Topological domain	1 – 37	37	Cytoplasmic By similarity
	Transmembrane	38 – 58	21	By similarity
	Topological domain	59 – 575	517	Extracellular By similarity
	Region	10 – 14	5	Incorporation in virion By similarity
	Region	59 – 140	82	Involved in interaction with F protein By similarity
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	499	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	511	1	N-linked (GlcNAc...); by host By similarity
	Disulfide bond	129		Interchain Potential
Natural variations
	Natural variant	123	1	R → Q in strain: Isolate vaccinal MVCES1.

Sequences

	Sequence		Length	Mass (Da)
	Q88261-1 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: 36AA3CD8BFF817D2	FASTA	575	63,238
	10 20 30 40 50 60 MDGDRSKRDS YWSTSPGGST TKLVSDSERS GKVDTWLLIL AFTQWALSIA TVIICIVIAA 70 80 90 100 110 120 RQGYSMERYS MTVEALNTSN KEVKESLTSL IRQEVITRAA NIQSSVQTGI PVLLNKNSRD 130 140 150 160 170 180 VIRLIEKSCN RQELTQLCDS TIAVHHAEGI APLEPHSFWR CPAGEPYLSS DPEVSLLPGP 190 200 210 220 230 240 SLLSGSTTIS GCVRLPSLSI GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD 250 260 270 280 290 300 LNPVVSHTYD INDNRKSCSV VATGTRGYQL CSMPIVDERT DYSSDGIEDL VLDILDLKGR 310 320 330 340 350 360 TKSHRYSNSE IDLDHPFSAL YPSVGSGIAT EGSLIFLGYG GLTTPLQGDT KCRIQGCQQV 370 380 390 400 410 420 SQDTCNEALK ITWLGGKQVV SVLIQVNDYL SERPRIRVTT IPITQNYLGA EGRLLKLGDQ 430 440 450 460 470 480 VYIYTRSSGW HSQLQIGVLD VSHPLTISWT PHEALSRPGN EDCNWYNTCP KECISGVYTD 490 500 510 520 530 540 AYPLSPDAAN VATVTLYANT SRVNPTIMYS NTTNIINMLR IKDVQLEAAY TTTSCITHFG 550 560 570 KGYCFHIIEI NQKSLNTLQP MLFKTSIPKL CKAES

References

[1]	"A protease activation mutant, MVCES1, as a safe and potent live vaccine derived from currently prevailing Sendai virus." Wang X.-L., Itoh M., Hotta H., Homma M. J. Virol. 68:3369-3373(1994) [PubMed: 8151795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate vaccinal MVCES1.
[2]	"Isolation of an avirulent mutant of Sendai virus with two amino acid mutations from a highly virulent field strain through adaptation to LLC-MK2 cells." Itoh M., Isegawa Y., Hotta H., Homma M. J. Gen. Virol. 78:3207-3215(1997) [PubMed: 9400971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate MVC11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26475 Genomic RNA. Translation: BAA05487.1. AB005795 Genomic RNA. Translation: BAA24391.1. AB005796 Genomic RNA. Translation: BAA24400.1.
RefSeq	NP_056878.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Genome annotation databases
GeneID	1489776.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_SENDO

Accession

Primary (citable) accession number: Q88261
Secondary accession number(s): O57288

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	April 12, 2005
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)