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Q88261

- HN_SENDO

UniProt

Q88261 - HN_SENDO

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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Sendai virus (strain Ohita) (SeV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Short name:
HN protein
Short name:
Protein HANA
Gene namesi
Name:HN
OrganismiSendai virus (strain Ohita) (SeV)
Taxonomic identifieri302272 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRespirovirus
Virus hostiCavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
ProteomesiUP000006563: Genome, UP000007311: Genome

Subcellular locationi

Virion membrane Curated; Single-pass type II membrane protein Curated. Host cell membrane Curated; Single-pass type II membrane protein Curated
Note: Folded in the endoplasmic reticulum.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737IntravirionBy similarityAdd
BLAST
Transmembranei38 – 5821HelicalBy similarityAdd
BLAST
Topological domaini59 – 575517Virion surfaceBy similarityAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Hemagglutinin-neuraminidasePRO_0000142639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...); by hostBy similarity
Disulfide bondi129 – 129InterchainSequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...); by hostBy similarity
Glycosylationi511 – 5111N-linked (GlcNAc...); by hostBy similarity

Post-translational modificationi

N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer; composed of disulfide-linked homodimers. Interacts with F protein trimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ88261.
SMRiQ88261. Positions 144-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 145Incorporation in virionBy similarity
Regioni59 – 14082Involved in interaction with F proteinBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88261-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGDRSKRDS YWSTSPGGST TKLVSDSERS GKVDTWLLIL AFTQWALSIA
60 70 80 90 100
TVIICIVIAA RQGYSMERYS MTVEALNTSN KEVKESLTSL IRQEVITRAA
110 120 130 140 150
NIQSSVQTGI PVLLNKNSRD VIRLIEKSCN RQELTQLCDS TIAVHHAEGI
160 170 180 190 200
APLEPHSFWR CPAGEPYLSS DPEVSLLPGP SLLSGSTTIS GCVRLPSLSI
210 220 230 240 250
GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD LNPVVSHTYD
260 270 280 290 300
INDNRKSCSV VATGTRGYQL CSMPIVDERT DYSSDGIEDL VLDILDLKGR
310 320 330 340 350
TKSHRYSNSE IDLDHPFSAL YPSVGSGIAT EGSLIFLGYG GLTTPLQGDT
360 370 380 390 400
KCRIQGCQQV SQDTCNEALK ITWLGGKQVV SVLIQVNDYL SERPRIRVTT
410 420 430 440 450
IPITQNYLGA EGRLLKLGDQ VYIYTRSSGW HSQLQIGVLD VSHPLTISWT
460 470 480 490 500
PHEALSRPGN EDCNWYNTCP KECISGVYTD AYPLSPDAAN VATVTLYANT
510 520 530 540 550
SRVNPTIMYS NTTNIINMLR IKDVQLEAAY TTTSCITHFG KGYCFHIIEI
560 570
NQKSLNTLQP MLFKTSIPKL CKAES
Length:575
Mass (Da):63,238
Last modified:April 12, 2005 - v2
Checksum:i36AA3CD8BFF817D2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti123 – 1231R → Q in strain: Isolate vaccinal MVCES1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26475 Genomic RNA. Translation: BAA05487.1.
AB005795 Genomic RNA. Translation: BAA24391.1.
AB005796 Genomic RNA. Translation: BAA24400.1.
RefSeqiNP_056878.1. NC_001552.1.

Genome annotation databases

GeneIDi1489776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26475 Genomic RNA. Translation: BAA05487.1 .
AB005795 Genomic RNA. Translation: BAA24391.1 .
AB005796 Genomic RNA. Translation: BAA24400.1 .
RefSeqi NP_056878.1. NC_001552.1.

3D structure databases

ProteinModelPortali Q88261.
SMRi Q88261. Positions 144-572.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1489776.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view ]
Pfami PF00423. HN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A protease activation mutant, MVCES1, as a safe and potent live vaccine derived from currently prevailing Sendai virus."
    Wang X.-L., Itoh M., Hotta H., Homma M.
    J. Virol. 68:3369-3373(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate vaccinal MVCES1.
  2. "Isolation of an avirulent mutant of Sendai virus with two amino acid mutations from a highly virulent field strain through adaptation to LLC-MK2 cells."
    Itoh M., Isegawa Y., Hotta H., Homma M.
    J. Gen. Virol. 78:3207-3215(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate MVC11.

Entry informationi

Entry nameiHN_SENDO
AccessioniPrimary (citable) accession number: Q88261
Secondary accession number(s): O57288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3