Reviewed, UniProtKB/Swiss-Prot Q881T7 (DNAE2_PSESM)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PSPTO_2795

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1031 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1031	1031	Error-prone DNA polymerase		PRO_0000103392

Sequences

	Sequence		Length	Mass (Da)
	Q881T7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 146574D77567B9D9	FASTA	1,031	115,990
	10 20 30 40 50 60 MNTEYAELHC LSNFSFQRGA SSARELFDRA LRHGYAALAI TDECTLAGIV RAWQASKDTG 70 80 90 100 110 120 LPLIVGSEMH LENGPKIVLL VENLTGYQAL CTLITVARRR AKKGEYRLLL EDFDHLPGGL 130 140 150 160 170 180 LAIWLADIEG DAQACLAQGN WLRERFAERL WLGVELHRGA DDEQRLADLL ALAQSLAIPA 190 200 210 220 230 240 VASGDVHMHA RGRRALQDTI TAIRHHTTVA EAGHLLFANG ERHLRSLDAL AEHYPDWLLA 250 260 270 280 290 300 ESLRIARRCT FTFDQLQYEY PHELVPKGQT STSWLRELTE RGIRRRWPRG LSVAARAQVE 310 320 330 340 350 360 KELALITEKK FDSYFLTVHD IVEFARSQHI LCQGRGSAAN SAVCYALGIT ELNPEKSNLL 370 380 390 400 410 420 FERFISKERD EPPDIDVDFE HDRREEVIQY IFRRYGRGRA ALTAVASTYH GSGALRDVAK 430 440 450 460 470 480 VLGLPPDQIN ALADAFSRWS DSLPSPERLR EYGFDAETPI LKRVLALTGE LIGFPRHLSQ 490 500 510 520 530 540 HPGGFVISEH PLDTLVPVEN AAMAERTIIQ WDKDDLDLVG LLKVDILALG MLSALRRTFD 550 560 570 580 590 600 LVHLHRGKQW TLASLPGDDR PTYEMISRAD TIGVFQIESR AQMAMLPRLR PEKFYDLVIE 610 620 630 640 650 660 VAIVRPGPIQ GDMVHPYLRR RNKEEAITYP PKLKSVFERT LGVPLFQEQV MEVAIIAADY 670 680 690 700 710 720 TPGEADQLRR AMAAWKRHGG LDPHRERLRT GMLANGYDAD FADRIFEQIK GFGSYGFPES 730 740 750 760 770 780 HAASFALLTY ASCWLKCHEP AAFTCALINS WPMGFYSPDQ LLQDARRHHI EIRPVDVRYS 790 800 810 820 830 840 HWDCTLEPLD HPDSTRNLAI RLGLRMLRSF REEDALRIEA ARSKRPFADA TDLAQRAGLD 850 860 870 880 890 900 SRAAEALADS GALRGLIGHR HRARWEVAGV EAQRPLFDDV TSEEVQVTLP LPTVAEDLVA 910 920 930 940 950 960 DYATLGTTLG PHPLALLRRQ LAAKRFRSSQ DLLSLENNRT LSVAGLVIGR QRPGTASGVT 970 980 990 1000 1010 1020 FVTLEDEFGM VNVVVWRDLA ERQRKVLVGS QLLQVFGRLE SNNGVRHLIA QRLYDLTPLL 1030 TGLEVRSRDF Q

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016853 Genomic DNA. Translation: AAO56295.1.
RefSeq	NP_792600.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1184449.
GenomeReviews	Gene locus PSPTO_2795 in contig AE016853_GR.
KEGG	pst:PSPTO_2795.
NMPDR	fig|223283.1.peg.2738.
TIGR	PSPTO_2795.
Phylogenomic databases
HOGENOM	Q881T7.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_2795-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSESM

Accession

Primary (citable) accession number: Q881T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)