NADH-quinone oxidoreductase subunit G - Pseudomonas syringae pv. tomato (strain DC3000)

Preferred order of sections

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit G
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	PSPTO_3370

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD⁺ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 3 4Fe-4S clusters per subunit By similarity.
Subunit structure	Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 905

905

NADH-quinone oxidoreductase subunit G

PRO_0000118552

Regions

Domain

1 – 83

83

2Fe-2S ferredoxin-type

Sites

Metal binding

34

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

45

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

48

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

67

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

99

1

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

103

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

106

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

112

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

151

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

154

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

157

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

201

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

228

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

231

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

235

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

263

1

Iron-sulfur 4 (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q87ZQ4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 53716244354E40DD
Show »

FASTA

905

98,334

        10         20         30         40         50         60 
MATIHVDGKA LEVDGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV KQYTDENDTR 

        70         80         90        100        110        120 
GRIVMSCMTP ATDNTWISIE DDESKAFRAS VVEWLMTNHP HDCPVCEEGG HCHLQDMTVM 

       130        140        150        160        170        180 
TGHNERRYRF TKRTHQNQEL GPFIAHEMNR CIACYRCVRF YKDYAGGTDL GVYGAHDNVY 

       190        200        210        220        230        240 
FGRVEDGTLE SEFSGNLTEV CPTGVFTDKT HSERYNRKWD MQFSPSICHG CSSGCNISPG 

       250        260        270        280        290        300 
ERYGELRRIE NRYNGSVNQY FLCDRGRFGY GYVNRTDRPR QPLLVDGQVK LSLDEALDKA 

       310        320        330        340        350        360 
ADLLRGRNIV GIGSPRASLE SNYALRELVG AEHFYSGIEA GELERVRLIL KVLNDSPLPI 

       370        380        390        400        410        420 
PTLRDIEDHD AIFVLGEDLT QTAARMALGL RQAVKGKAED MAAAMKVQPW LDAAVKNIGQ 

       430        440        450        460        470        480 
HELNPLFIAS LAETRLDDIA EECVHAAPDD LARIGFAVAH ALDASAPAVE GLDSEALELA 

       490        500        510        520        530        540 
QRIADALLAA KRPLIISGSS LGSTALIEAA ANIAKALKLR DKQGSISLIV PEANSMGLAM 

       550        560        570        580        590        600 
FGGDSVDAAL QAVISGKADA IVVLENDLYR RVDAKIVDAA LSAAKVVIVA DHQKTPTSDR 

       610        620        630        640        650        660 
AHLVLPAASF AEGDGTLVSQ EGRAQRFFQV FDPTYLDASI LVHEGWRWLH ALRATLLNKP 

       670        680        690        700        710        720 
VDWTQLDHVT EACAGSTAQL AGIVNAAPSA SFRIKGLKLA REPLRYSGRT AMRANISVHE 

       730        740        750        760        770        780 
PRTSQDIDTA FSFSMEGYSG SAEPRQQVPF AWSPGWNSPQ AWNKFQDEVG GHLRAGDPGT 

       790        800        810        820        830        840 
RLIESQGDGL SWFSSVPRAF SPERGTWQVV PFFHLFGSDE TSSLAAPVQE RIPQAYVAVA 

       850        860        870        880        890        900 
KSEADRLGVN DGALLSLSIG GETLTLPLRI SEELGAGLVA LPAGLPGIPP ALFGKSVDGL 


QEAAQ

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References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000."
Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N.

Collmer A.
Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO56848.1.
RefSeq	NP_793153.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q87ZQ4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1185029.
GenomeReviews	Gene locus PSPTO_3370 in contig AE016853_GR.
KEGG	pst:PSPTO_3370.
NMPDR	fig\|223283.1.peg.3291.
PATRIC	19998146. VBIPseSyr93040_3450.
TIGR	PSPTO_3370.
Phylogenomic databases
HOGENOM	HBG488172.
OMA	PSICHGC.
ProtClustDB	PRK08166.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_3370-MONOMER.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR012675. Beta-grasp_ferredoxin-type. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K00336.
Pfam	PF00111. Fer2. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit. SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOG_PSESM

Accession

Primary (citable) accession number: Q87ZQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents