Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q87Z72 (MASZ2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G 2

EC=2.3.3.9
Gene names
Name:glcB2
Synonyms:glcB-2
Ordered Locus Names:PSPTO_3559
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity. HAMAP-Rule MF_00641

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Malate synthase G 2 HAMAP-Rule MF_00641
PRO_0000166895

Regions

Region125 – 1262Acetyl-CoA binding By similarity
Region454 – 4574Glyoxylate binding By similarity

Sites

Active site3401Proton acceptor By similarity
Active site6331Proton donor By similarity
Metal binding4291Magnesium By similarity
Metal binding4571Magnesium By similarity
Binding site1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding site2761Acetyl-CoA By similarity
Binding site3131Acetyl-CoA By similarity
Binding site3401Glyoxylate By similarity
Binding site4291Glyoxylate By similarity
Binding site5381Acetyl-CoA; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue6191Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87Z72 [UniParc].

Last modified April 30, 2003. Version 1.
Checksum: 8F5EA38DB371E7AE

FASTA72580,054
        10         20         30         40         50         60 
MTDFVKCQRL KVAVKLQRFV DEEVLPGTGL EPEAFWKGFD ALVHDLAPVN RELLAERERL 

        70         80         90        100        110        120 
QGQLDKWHKA HPGPITDMPA YRAFLHSIGY LQPVPEHVAA TTANVDLEIS EQAGPQLVVP 

       130        140        150        160        170        180 
ASNARYALNA ANARWGSLYD ALYGTDVIST SNGAEIRAGY NPLRGAKVIA FARELLDTSA 

       190        200        210        220        230        240 
PLANGSHSDA NRYRIEDGEL RITLADGSQT LLQHPEKYVG FTGEPDQPQA ILLKNHGLHI 

       250        260        270        280        290        300 
EIQFDPQHPV GKTDAAGIKD LLLESALSTI VDCEDSVAAV DADDKVLVYR NWLGLMKGDL 

       310        320        330        340        350        360 
TETLDKGGKA ITRSLNPDRV YHAAAGGELT LRGRSLLLIR NVGHLMSNPA IVDQDDKEIP 

       370        380        390        400        410        420 
EGILDGVITS LIGLHDLTRH GNSRAGSIYI VKPKMHGAAE VAFADQLFSR IEDVLHLPRN 

       430        440        450        460        470        480 
TLKMGIMDEE RRTSVNLKAC INAASARVAF INTGFLDRTG DEIHSAMQAG PVLRKGEMKN 

       490        500        510        520        530        540 
TPWIKAYERN NVQVGLACGL RGRAQIGKGM WAMPDRMADM LEQKIAHPKS GATTAWVPSP 

       550        560        570        580        590        600 
TAATLHAWHY HHVDVRQVQE QLEQQSLVDV SDQWLDDLLT IPVVGKPEWT REQILEELEN 

       610        620        630        640        650        660 
NTQGLLGYVV RWVEQGIGCS KVPDIHNVGL MEDRATLRIS SQHMANWLLH DIAGKEDIQN 

       670        680        690        700        710        720 
VLERMAKVVD KQNAGDSHYR PMTRDFKNSA AFKAACALVF KGTEQPSGYT EPLLHESRLA 


FKDAH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016853 Genomic DNA. Translation: AAO57033.1.
RefSeqNP_793338.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ87Z72.
SMRQ87Z72. Positions 1-725.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223283.PSPTO_3559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO57033; AAO57033; PSPTO_3559.
GeneID1185224.
KEGGpst:PSPTO_3559.
PATRIC19998556. VBIPseSyr93040_3649.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000220740.
KOK01638.
OMAPANTLKM.
OrthoDBEOG6HJ286.
PhylomeDBQ87Z72.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-3616-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ2_PSESM
AccessionPrimary (citable) accession number: Q87Z72
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways