Q87Z72 (MASZ2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G 2
EC=2.3.3.9

Gene names

Name:	glcB2
Synonyms:	glcB-2
Ordered Locus Names:	PSPTO_3559

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 3 › Pseudomonas syringae pv. tomato

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G 2 HAMAP-Rule MF_00641		PRO_0000166895
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	633	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87Z72 [UniParc]. Last modified April 30, 2003. Version 1. Checksum: 8F5EA38DB371E7AE	FASTA	725	80,054
	10 20 30 40 50 60 MTDFVKCQRL KVAVKLQRFV DEEVLPGTGL EPEAFWKGFD ALVHDLAPVN RELLAERERL 70 80 90 100 110 120 QGQLDKWHKA HPGPITDMPA YRAFLHSIGY LQPVPEHVAA TTANVDLEIS EQAGPQLVVP 130 140 150 160 170 180 ASNARYALNA ANARWGSLYD ALYGTDVIST SNGAEIRAGY NPLRGAKVIA FARELLDTSA 190 200 210 220 230 240 PLANGSHSDA NRYRIEDGEL RITLADGSQT LLQHPEKYVG FTGEPDQPQA ILLKNHGLHI 250 260 270 280 290 300 EIQFDPQHPV GKTDAAGIKD LLLESALSTI VDCEDSVAAV DADDKVLVYR NWLGLMKGDL 310 320 330 340 350 360 TETLDKGGKA ITRSLNPDRV YHAAAGGELT LRGRSLLLIR NVGHLMSNPA IVDQDDKEIP 370 380 390 400 410 420 EGILDGVITS LIGLHDLTRH GNSRAGSIYI VKPKMHGAAE VAFADQLFSR IEDVLHLPRN 430 440 450 460 470 480 TLKMGIMDEE RRTSVNLKAC INAASARVAF INTGFLDRTG DEIHSAMQAG PVLRKGEMKN 490 500 510 520 530 540 TPWIKAYERN NVQVGLACGL RGRAQIGKGM WAMPDRMADM LEQKIAHPKS GATTAWVPSP 550 560 570 580 590 600 TAATLHAWHY HHVDVRQVQE QLEQQSLVDV SDQWLDDLLT IPVVGKPEWT REQILEELEN 610 620 630 640 650 660 NTQGLLGYVV RWVEQGIGCS KVPDIHNVGL MEDRATLRIS SQHMANWLLH DIAGKEDIQN 670 680 690 700 710 720 VLERMAKVVD KQNAGDSHYR PMTRDFKNSA AFKAACALVF KGTEQPSGYT EPLLHESRLA FKDAH

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO57033.1.
RefSeq	NP_793338.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q87Z72.
SMR	Q87Z72. Positions 1-725.
ModBase	Search...
Protein-protein interaction databases
STRING	223283.PSPTO_3559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO57033; AAO57033; PSPTO_3559.
GeneID	1185224.
KEGG	pst:PSPTO_3559.
PATRIC	19998556. VBIPseSyr93040_3649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	SQFIENE.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	PSYR223283:GJIX-3616-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ2_PSESM

Accession

Primary (citable) accession number: Q87Z72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program