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Protein

Malate synthase G 2

Gene

glcB2

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G 1 (glcB1), Malate synthase G 2 (glcB2)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei633Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G 2UniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcB2UniRule annotation
Synonyms:glcB-2
Ordered Locus Names:PSPTO_3559
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668951 – 725Malate synthase G 2Add BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei619Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_3559.

Structurei

3D structure databases

ProteinModelPortaliQ87Z72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105TVE. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiGYNEVRG.
OrthoDBiPOG091H05P0.
PhylomeDBiQ87Z72.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87Z72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDFVKCQRL KVAVKLQRFV DEEVLPGTGL EPEAFWKGFD ALVHDLAPVN
60 70 80 90 100
RELLAERERL QGQLDKWHKA HPGPITDMPA YRAFLHSIGY LQPVPEHVAA
110 120 130 140 150
TTANVDLEIS EQAGPQLVVP ASNARYALNA ANARWGSLYD ALYGTDVIST
160 170 180 190 200
SNGAEIRAGY NPLRGAKVIA FARELLDTSA PLANGSHSDA NRYRIEDGEL
210 220 230 240 250
RITLADGSQT LLQHPEKYVG FTGEPDQPQA ILLKNHGLHI EIQFDPQHPV
260 270 280 290 300
GKTDAAGIKD LLLESALSTI VDCEDSVAAV DADDKVLVYR NWLGLMKGDL
310 320 330 340 350
TETLDKGGKA ITRSLNPDRV YHAAAGGELT LRGRSLLLIR NVGHLMSNPA
360 370 380 390 400
IVDQDDKEIP EGILDGVITS LIGLHDLTRH GNSRAGSIYI VKPKMHGAAE
410 420 430 440 450
VAFADQLFSR IEDVLHLPRN TLKMGIMDEE RRTSVNLKAC INAASARVAF
460 470 480 490 500
INTGFLDRTG DEIHSAMQAG PVLRKGEMKN TPWIKAYERN NVQVGLACGL
510 520 530 540 550
RGRAQIGKGM WAMPDRMADM LEQKIAHPKS GATTAWVPSP TAATLHAWHY
560 570 580 590 600
HHVDVRQVQE QLEQQSLVDV SDQWLDDLLT IPVVGKPEWT REQILEELEN
610 620 630 640 650
NTQGLLGYVV RWVEQGIGCS KVPDIHNVGL MEDRATLRIS SQHMANWLLH
660 670 680 690 700
DIAGKEDIQN VLERMAKVVD KQNAGDSHYR PMTRDFKNSA AFKAACALVF
710 720
KGTEQPSGYT EPLLHESRLA FKDAH
Length:725
Mass (Da):80,054
Last modified:April 30, 2003 - v1
Checksum:i8F5EA38DB371E7AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57033.1.
RefSeqiNP_793338.1. NC_004578.1.
WP_005765147.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO57033; AAO57033; PSPTO_3559.
GeneIDi1185224.
KEGGipst:PSPTO_3559.
PATRICi19998556. VBIPseSyr93040_3649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57033.1.
RefSeqiNP_793338.1. NC_004578.1.
WP_005765147.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ87Z72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_3559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO57033; AAO57033; PSPTO_3559.
GeneIDi1185224.
KEGGipst:PSPTO_3559.
PATRICi19998556. VBIPseSyr93040_3649.

Phylogenomic databases

eggNOGiENOG4105TVE. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiGYNEVRG.
OrthoDBiPOG091H05P0.
PhylomeDBiQ87Z72.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ2_PSESM
AccessioniPrimary (citable) accession number: Q87Z72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.