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Q87Z72

- MASZ2_PSESM

UniProt

Q87Z72 - MASZ2_PSESM

Protein

Malate synthase G 2

Gene

glcB2

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (30 Apr 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei633 – 6331Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-3616-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase G 2UniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcB2UniRule annotation
    Synonyms:glcB-2
    Ordered Locus Names:PSPTO_3559
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002515: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase G 2PRO_0000166895Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei619 – 6191Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi223283.PSPTO_3559.

    Structurei

    3D structure databases

    ProteinModelPortaliQ87Z72.
    SMRiQ87Z72. Positions 1-725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiPANTLKM.
    OrthoDBiEOG6HJ286.
    PhylomeDBiQ87Z72.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q87Z72-1 [UniParc]FASTAAdd to Basket

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    MTDFVKCQRL KVAVKLQRFV DEEVLPGTGL EPEAFWKGFD ALVHDLAPVN    50
    RELLAERERL QGQLDKWHKA HPGPITDMPA YRAFLHSIGY LQPVPEHVAA 100
    TTANVDLEIS EQAGPQLVVP ASNARYALNA ANARWGSLYD ALYGTDVIST 150
    SNGAEIRAGY NPLRGAKVIA FARELLDTSA PLANGSHSDA NRYRIEDGEL 200
    RITLADGSQT LLQHPEKYVG FTGEPDQPQA ILLKNHGLHI EIQFDPQHPV 250
    GKTDAAGIKD LLLESALSTI VDCEDSVAAV DADDKVLVYR NWLGLMKGDL 300
    TETLDKGGKA ITRSLNPDRV YHAAAGGELT LRGRSLLLIR NVGHLMSNPA 350
    IVDQDDKEIP EGILDGVITS LIGLHDLTRH GNSRAGSIYI VKPKMHGAAE 400
    VAFADQLFSR IEDVLHLPRN TLKMGIMDEE RRTSVNLKAC INAASARVAF 450
    INTGFLDRTG DEIHSAMQAG PVLRKGEMKN TPWIKAYERN NVQVGLACGL 500
    RGRAQIGKGM WAMPDRMADM LEQKIAHPKS GATTAWVPSP TAATLHAWHY 550
    HHVDVRQVQE QLEQQSLVDV SDQWLDDLLT IPVVGKPEWT REQILEELEN 600
    NTQGLLGYVV RWVEQGIGCS KVPDIHNVGL MEDRATLRIS SQHMANWLLH 650
    DIAGKEDIQN VLERMAKVVD KQNAGDSHYR PMTRDFKNSA AFKAACALVF 700
    KGTEQPSGYT EPLLHESRLA FKDAH 725
    Length:725
    Mass (Da):80,054
    Last modified:April 30, 2003 - v1
    Checksum:i8F5EA38DB371E7AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO57033.1.
    RefSeqiNP_793338.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO57033; AAO57033; PSPTO_3559.
    GeneIDi1185224.
    KEGGipst:PSPTO_3559.
    PATRICi19998556. VBIPseSyr93040_3649.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016853 Genomic DNA. Translation: AAO57033.1 .
    RefSeqi NP_793338.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q87Z72.
    SMRi Q87Z72. Positions 1-725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_3559.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO57033 ; AAO57033 ; PSPTO_3559 .
    GeneIDi 1185224.
    KEGGi pst:PSPTO_3559.
    PATRICi 19998556. VBIPseSyr93040_3649.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi PANTLKM.
    OrthoDBi EOG6HJ286.
    PhylomeDBi Q87Z72.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci PSYR223283:GJIX-3616-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.

    Entry informationi

    Entry nameiMASZ2_PSESM
    AccessioniPrimary (citable) accession number: Q87Z72
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3