Reviewed, UniProtKB/Swiss-Prot Q87YQ1 (SYQ_PSESM)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	PSPTO_3743

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195845
Regions
	Motif	50 – 60	11	"HIGH" region HAMAP MF_00126
	Motif	284 – 288	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	287	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87YQ1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C50C8478CD102CA1	FASTA	569	65,197
	10 20 30 40 50 60 MSKDPMSKPT PEPAAHSKAG PAVPTNFLRP IVQADLDSGK HSKIITRFPP EPNGYLHIGH 70 80 90 100 110 120 AKSICVNFGL AKEFGGDTHL RFDDTNPAKE DQEYIDAIMN DVKWLGFEWA GEVRYASQYF 130 140 150 160 170 180 DQLHDWAVEL IKAGKAYVDD LTPEQAREYR GTLTEPGKNS PFRERSVEEN IDLFARMKAG 190 200 210 220 230 240 EFEDGARVLR AKIDMASPNM NLRDPILYRI RHAHHHQTGD KWCIYPIYDF THGQSDAIEG 250 260 270 280 290 300 ITHSICTLEF ESHRPLYDWF LDNLPVPCKP RQYEFSRLNL NYTITSKRKL KQLVDEKHVN 310 320 330 340 350 360 GWDDPRMSTL SGFRRRGYTP KSIRNFCEMI GTNRSDGVVD FGMLEFSIRD DLDHSAPRAM 370 380 390 400 410 420 CVLRPLKVVI TNYPEGQVEK LELPRHPKED LGMRELPFSR EIYIDRDDYM EEPPKGYKRL 430 440 450 460 470 480 EPNGEVRLRG SYVIRADEAI KDADGNIVEL RCSYDPDTLG KNPEGRKVKG VVHWVPAAES 490 500 510 520 530 540 VECEVRLYDR LFRSANPEKA EDGASFLDNI NPDSLQVLTG CRAEPSLGHA QPEDRFQFER 550 560 EGYFCADIKD SKPGQPVFNR TVTLRDSWT

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO57212.1.
RefSeq	NP_793517.1.
3D structure databases
SMR	Q87YQ1. Positions 25-567.
ModBase	Search...
Genome annotation databases
GeneID	1185411.
GenomeReviews	Gene locus PSPTO_3743 in contig AE016853_GR.
KEGG	pst:PSPTO_3743.
NMPDR	fig|223283.1.peg.3655.
TIGR	PSPTO_3743.
Phylogenomic databases
HOGENOM	HBG334108.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_3743-MONOMER.
BRENDA	6.1.1.18. 289554.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PSESM

Accession

Primary (citable) accession number: Q87YQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)