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Q87Y33

- RUVC_PSESM

UniProt

Q87Y33 - RUVC_PSESM

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Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, PSPTO_3979
Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium By similarity
Metal bindingi69 – 691Magnesium By similarity
Metal bindingi141 – 1411Magnesium By similarity
Metal bindingi144 – 1441Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-4044-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:PSPTO_3979
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_0000183123Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_3979.

Structurei

3D structure databases

ProteinModelPortaliQ87Y33.
SMRiQ87Y33. Positions 2-160.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiRQVQEMV.
OrthoDBiEOG6RG044.
PhylomeDBiQ87Y33.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q87Y33-1 [UniParc]FASTAAdd to Basket

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MTLILGIDPG SRITGYGVVR DTGRGCVYVA SGCIRTGTGS GELHERLQIV    50
YRGVREVIKT YGPVTMGIEK VFMARNADSA LKLGQARGAA IVAGAEEALE 100
IAEYTATQVK QAVAGTGGAN KEQVMMMVMH LLKLTQKPQI DASDALAIAL 150
CHAHTRSSLI PHGLGTARSR GGRLRL 176
Length:176
Mass (Da):18,602
Last modified:June 1, 2003 - v1
Checksum:i35E886C75BA61F64
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016853 Genomic DNA. Translation: AAO57438.1.
RefSeqiNP_793743.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO57438; AAO57438; PSPTO_3979.
GeneIDi1185655.
KEGGipst:PSPTO_3979.
PATRICi19999435. VBIPseSyr93040_4079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016853 Genomic DNA. Translation: AAO57438.1 .
RefSeqi NP_793743.1. NC_004578.1.

3D structure databases

ProteinModelPortali Q87Y33.
SMRi Q87Y33. Positions 2-160.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223283.PSPTO_3979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO57438 ; AAO57438 ; PSPTO_3979 .
GeneIDi 1185655.
KEGGi pst:PSPTO_3979.
PATRICi 19999435. VBIPseSyr93040_4079.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi RQVQEMV.
OrthoDBi EOG6RG044.
PhylomeDBi Q87Y33.

Enzyme and pathway databases

BioCyci PSYR223283:GJIX-4044-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiRUVC_PSESM
AccessioniPrimary (citable) accession number: Q87Y33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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