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Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Magnesium or manganese 1UniRule annotation1
Metal bindingi312Magnesium or manganese 1UniRule annotation1
Metal bindingi312Magnesium or manganese 2UniRule annotation1
Metal bindingi314Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 227ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-4247-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase AUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligase AUniRule annotation
D-alanylalanine synthetase AUniRule annotation
Gene namesi
Name:ddlAUniRule annotation
Ordered Locus Names:PSPTO_4182
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778591 – 363D-alanine--D-alanine ligase AAdd BLAST363

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_4182

Structurei

3D structure databases

ProteinModelPortaliQ87XJ6
SMRiQ87XJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 345ATP-graspUniRule annotationAdd BLAST202

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiRCDFFVI
OrthoDBiPOG091H06GK
PhylomeDBiQ87XJ6

Family and domain databases

Gene3Di3.30.1490.201 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q87XJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSVAIVFG GQSSEHEVSL QSARNVINAI DRESYALTLI GVDKLGRWLR
60 70 80 90 100
FDEADYLLNA NDPARIKLSG SGKQLSLLPG SVGGQFVEVE SGKPLAAIDV
110 120 130 140 150
VFPLIHGAFG EDGALQGLLR MLAVPFVGAD VLASAACMDK DVTKRLLRDA
160 170 180 190 200
GIAVAPFMVL NRGESLSFAQ AAAQLSLPMF VKPANQGSSV GVSKVSDEAG
210 220 230 240 250
FAAALALGFE FDHKLLIEQG IVGREVECAV LGNDEAQVSV CGEVVANDEF
260 270 280 290 300
YAYDTKYLNG DQARIAIPAE LPADLSDQVR DVALQAYKVL GCAGLSRVDF
310 320 330 340 350
FVTEAREIII NEVNTLPGFT SISMYPKLWQ ATGLSYAELI HRLIVLALER
360
ADEARLLKRD IFA
Length:363
Mass (Da):38,948
Last modified:June 1, 2003 - v1
Checksum:i8E9772ACDAB380FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO57638.1
RefSeqiNP_793943.1, NC_004578.1
WP_005764809.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO57638; AAO57638; PSPTO_4182
GeneIDi1185862
KEGGipst:PSPTO_4182
PATRICifig|223283.9.peg.4292

Similar proteinsi

Entry informationi

Entry nameiDDLA_PSESM
AccessioniPrimary (citable) accession number: Q87XJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome