UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase - Pseudomonas syringae pv. tomato (strain DC3000)

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Q87WY0 (MURE_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13

Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	PSPTO_4413

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg²⁺ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208
Sequence similarities	Belongs to the MurCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 487

487

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208

PRO_0000101928

Regions

Nucleotide binding

108 – 114

ATP Potential

Region

150 – 151

UDP-MurNAc-L-Ala-D-Glu binding By similarity

Region

402 – 405

Meso-diaminopimelate binding By similarity

Motif

402 – 405

Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208

Sites

Binding site

UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity

Binding site

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

177

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

183

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

185

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

378

Meso-diaminopimelate By similarity

Binding site

453

Meso-diaminopimelate; via carbonyl oxygen By similarity

Binding site

457

Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue

217

N6-carboxylysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q87WY0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: FBF47A42D387E905
Show »

FASTA

487

52,197

        10         20         30         40         50         60 
MAFNLSKIFA HTDRDPLIRE LTLDSRNVRP GDLFLAVPGI KVDGRAHIAD ALKRGAAAVA 

        70         80         90        100        110        120 
YEVEGATVLP ITDVPLIPVK GLAAQLSDVA GRFYGDPSRS LNLVGVTGTN GKTSVTQLVA 

       130        140        150        160        170        180 
QALDALGQRC GIVGTLGTGF YGALQSGRHT TPDPIAVQAA LTDLRKAGAR AVAMEVSSHG 

       190        200        210        220        230        240 
LDQGRATALA FDVAVLTNLS RDHLDYHGTM EAYAAAKAKL FAWPNLNCRV INLDDDFGRE 

       250        260        270        280        290        300 
LAGLKQESRL ITYSQLDSSA YLYCRDAKFD DDGVRATLVT PQGEHFLRSS LLGRFNLSNV 

       310        320        330        340        350        360 
LAAVGALLGL DYALDEILKV LPKLEGPVGR MQRLGGADKP LVVVDYAHTP DALEKVLEAL 

       370        380        390        400        410        420 
RPHAKGRLLC LFGCGGDRDR GKRPLMAEVV ERLADGVWVT DDNPRSEAPT NIFDDIRPGF 

       430        440        450        460        470        480 
VTADKVRFIE GRGQAIAELI ASATVDDVVV LAGKGHEDYQ EIDGKRQPFS DLEEAATALA 


AWGPEND

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References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000."
Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N.

Collmer A.
Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO57862.1.
RefSeq	NP_794167.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q87WY0.
ModBase	Search...
Protein-protein interaction databases
STRING	223283.PSPTO_4413.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO57862; AAO57862; PSPTO_4413.
GeneID	1186094.
KEGG	pst:PSPTO_4413.
PATRIC	20000336. VBIPseSyr93040_4528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HOG000268118.
KO	K01928.
OMA	FEDYLAC.
ProtClustDB	PRK00139.
Enzyme and pathway databases
BioCyc	PSYR223283:GJIX-4478-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00208. MurE.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_PSESM

Accession

Primary (citable) accession number: Q87WY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents