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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei207 – 2071ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-4495-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:PSPTO_4429
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Tryptophan--tRNA ligasePRO_0000136664Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_4429.

Structurei

3D structure databases

ProteinModelPortaliQ87WW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 199"HIGH" region
Motifi204 – 2085"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.
PhylomeDBiQ87WW4.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87WW4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTRILTGIT TTGTPHLGNY AGAIRPAIVA SRQSDVDSFY FLADYHALIK
60 70 80 90 100
CDDPLRIQRS RLEIAATWLA AGLDVDRVTF YRQSDIPEIP ELTWLLTCVA
110 120 130 140 150
AKGLLNRAHA YKASVDKNVE SCEDPDAGIT MGLYSYPVLM AADILMFNAH
160 170 180 190 200
QVPVGRDQIQ HVEMARDIGQ RFNHLFGKGR EFFVMPEALI EESVATLPGL
210 220 230 240 250
DGRKMSKSYD NTIPLFSSAK DMKSAISRIV TDSLAPGEAK DPDNSHLFTL
260 270 280 290 300
YQAFSTPEQC AQFRSELLQG LGWGEAKTRM FTLLDEQLGE AREQYLSLIE
310 320 330 340 350
RPADLEDILL AGAQKARRVA TPFLEELREA VGLRSFRTAV QNADTGKKKT
360 370 380 390 400
TKGARFVSFR EDDGSFRFRL LAADGEQLLL SRTFADGKAA GIVSKQLQQG
410 420 430 440
GELDLRSEAD RFTLWLNGEC VADSPVFADA TARDNAVETL KLALAPQQD
Length:449
Mass (Da):49,635
Last modified:June 1, 2003 - v1
Checksum:i7697DB7C99120F85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57878.1.
RefSeqiNP_794183.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO57878; AAO57878; PSPTO_4429.
GeneIDi1186110.
KEGGipst:PSPTO_4429.
PATRICi20000368. VBIPseSyr93040_4544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57878.1.
RefSeqiNP_794183.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ87WW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_4429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO57878; AAO57878; PSPTO_4429.
GeneIDi1186110.
KEGGipst:PSPTO_4429.
PATRICi20000368. VBIPseSyr93040_4544.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.
PhylomeDBiQ87WW4.

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-4495-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiSYW_PSESM
AccessioniPrimary (citable) accession number: Q87WW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.