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Protein

Sulfate adenylyltransferase subunit 1

Gene

C

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.SAAS annotation
ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotationSAAS annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (C)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388GTPUniRule annotation
Nucleotide bindingi110 – 1145GTPUniRule annotation
Nucleotide bindingi165 – 1684GTPUniRule annotation

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: JCVI
  • ATP binding Source: UniProtKB-KW
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-HAMAP
  • sulfate adenylyltransferase (ATP) activity Source: JCVI

GO - Biological processi

  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • sulfate assimilation Source: UniProtKB-HAMAP
  • sulfur compound metabolic process Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

KinaseSAAS annotationImported, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, GTP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-4498-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Gene namesi
Name:CImported
Synonyms:cysNUniRule annotationImported
Ordered Locus Names:PSPTO_4432Imported
OrganismiPseudomonas syringae pv. tomato (strain DC3000)Imported
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_4432.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUNX-ray2.70B1-434[»]
ProteinModelPortaliQ87WW1.
SMRiQ87WW1. Positions 16-434.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ87WW1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 241220Tr-type G (guanine nucleotide-binding)InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation
Contains tr-type G (guanine nucleotide-binding) domain.SAAS annotation

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG0529. LUCA.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00955.
OMAiQRDSRKH.
OrthoDBiPOG091H06C6.
PhylomeDBiQ87WW1.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR002891. APS_kinase.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q87WW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQSDLISE DILAYLGQHE RKEMLRFLTC GNVDDGKSTL IGRLLHDSKM
60 70 80 90 100
IYEDHLEAIT RDSKKSGTTG DDVDLALLVD GLQAEREQGI TIDVAYRYFS
110 120 130 140 150
TAKRKFIIAD TPGHEQYTRN MATGASTCDL AIILVDARYG VQTQTRRHSY
160 170 180 190 200
IASLLGIKHI VVAINKMDLN GFDERVFESI KADYLKFAEG IAFKPTTMAF
210 220 230 240 250
VPMSALKGDN VVNKSERSPW YAGQSLMEIL ETVEIASDRN YTDLRFPVQY
260 270 280 290 300
VNRPNLNFRG FAGTLASGIV HKGDEIVVLP SGKSSRVKSI VTFEGELEQA
310 320 330 340 350
GPGQAVTLTM EDEIDISRGD LLVHADNVPQ VSDAFDAMLV WMAEEPMLPG
360 370 380 390 400
KKYDIKRATS YVPGSIASIT HRVDVNTLEE GPASSLQLNE IGRVKVSLDA
410 420 430 440 450
PIALDGYSSN RTTGAFIVID RLTNGTVAAG MIIAKPVSGG GSHHHGELAH
460 470 480 490 500
VSTEERAQRF GQQPATVLFS GLSGAGKSTL AYAVERKLFD MGRAVYVLDG
510 520 530 540 550
QNLRHDLNKG LPQDRAGRTE NWSRAAHVAR QFNEAGLLTL AAFVAPDAEG
560 570 580 590 600
RERAKALIGK ERLVTVYVQA SPAVCRERDP QGLYAADGDN IPGESFPYDV
610 620 630
PLNADLVIDT QTLSVEESVK QVLDLLRGRG AI
Length:632
Mass (Da):69,053
Last modified:June 1, 2003 - v1
Checksum:i4EE729FBD73B23E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57881.1.
RefSeqiNP_794186.1. NC_004578.1.
WP_011105005.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO57881; AAO57881; PSPTO_4432.
GeneIDi1186113.
KEGGipst:PSPTO_4432.
PATRICi20000374. VBIPseSyr93040_4547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57881.1.
RefSeqiNP_794186.1. NC_004578.1.
WP_011105005.1. NC_004578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUNX-ray2.70B1-434[»]
ProteinModelPortaliQ87WW1.
SMRiQ87WW1. Positions 16-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_4432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO57881; AAO57881; PSPTO_4432.
GeneIDi1186113.
KEGGipst:PSPTO_4432.
PATRICi20000374. VBIPseSyr93040_4547.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG0529. LUCA.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00955.
OMAiQRDSRKH.
OrthoDBiPOG091H06C6.
PhylomeDBiQ87WW1.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciPSYR223283:GJIX-4498-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ87WW1.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR002891. APS_kinase.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ87WW1_PSESM
AccessioniPrimary (citable) accession number: Q87WW1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.