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Protein

Sulfate adenylyltransferase subunit 2

Gene

cysD

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (C)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • sulfate adenylyltransferase (ATP) activity Source: JCVI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 2UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase small subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysDUniRule annotation
Ordered Locus Names:PSPTO_4433
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006711 – 305Sulfate adenylyltransferase subunit 2Add BLAST305

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_4433.

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 25Combined sources19
Beta strandi27 – 32Combined sources6
Helixi37 – 50Combined sources14
Beta strandi59 – 62Combined sources4
Helixi69 – 80Combined sources12
Turni81 – 83Combined sources3
Beta strandi86 – 89Combined sources4
Helixi108 – 113Combined sources6
Helixi115 – 125Combined sources11
Beta strandi128 – 131Combined sources4
Helixi140 – 144Combined sources5
Beta strandi147 – 151Combined sources5
Helixi159 – 161Combined sources3
Beta strandi179 – 182Combined sources4
Turni184 – 187Combined sources4
Helixi190 – 200Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZUNX-ray2.70A1-305[»]
ProteinModelPortaliQ87WW0.
SMRiQ87WW0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ87WW0.

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C11. Bacteria.
COG0175. LUCA.
HOGENOMiHOG000263604.
KOiK00957.
OMAiQSASMEK.
OrthoDBiPOG091H03PV.
PhylomeDBiQ87WW0.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87WW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDKLTHLKQ LEAESIHIIR EVAAEFDNPV MLYSIGKDSA VMLHLARKAF
60 70 80 90 100
FPGKLPFPVM HVDTRWKFQE MYRFRDQMVE EMGLDLITHI NPDGVAQGIN
110 120 130 140 150
PFTHGSAKHT DIMKTEGLKQ ALDKHGFDAA FGGARRDEEK SRAKERVYSF
160 170 180 190 200
RDSKHRWDPK NQRPELWNVY NGNVNKGESI RVFPLSNWTE LDIWQYIYLE
210 220 230 240 250
GIPIVPLYFA AERDVIEKNG TLIMIDDERI LEHLTDEEKS RIVKKKVRFR
260 270 280 290 300
TLGCYPLTGA VESEATSLTD IIQEMLLTRT SERQGRVIDH DGAGSMEEKK

RQGYF
Length:305
Mass (Da):35,312
Last modified:June 1, 2003 - v1
Checksum:iC34C7ECA9448552F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57882.1.
RefSeqiNP_794187.1. NC_004578.1.
WP_003313577.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO57882; AAO57882; PSPTO_4433.
GeneIDi1186114.
KEGGipst:PSPTO_4433.
PATRICi20000376. VBIPseSyr93040_4548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO57882.1.
RefSeqiNP_794187.1. NC_004578.1.
WP_003313577.1. NC_004578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZUNX-ray2.70A1-305[»]
ProteinModelPortaliQ87WW0.
SMRiQ87WW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_4433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO57882; AAO57882; PSPTO_4433.
GeneIDi1186114.
KEGGipst:PSPTO_4433.
PATRICi20000376. VBIPseSyr93040_4548.

Phylogenomic databases

eggNOGiENOG4105C11. Bacteria.
COG0175. LUCA.
HOGENOMiHOG000263604.
KOiK00957.
OMAiQSASMEK.
OrthoDBiPOG091H03PV.
PhylomeDBiQ87WW0.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Miscellaneous databases

EvolutionaryTraceiQ87WW0.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSD_PSESM
AccessioniPrimary (citable) accession number: Q87WW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.