Q87WW0 (CYSD_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	PSPTO_4433

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 305

305

Sulfate adenylyltransferase subunit 2 HAMAP MF_00064

PRO_0000100671

Secondary structure

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305

Helix Strand Turn

Details...

Helix

7 – 25

19

Beta strand

27 – 32

6

Helix

37 – 50

14

Beta strand

59 – 62

4

Helix

69 – 80

12

Turn

81 – 83

3

Beta strand

86 – 89

4

Helix

108 – 113

6

Helix

115 – 125

11

Beta strand

128 – 131

4

Helix

140 – 144

5

Beta strand

147 – 151

5

Helix

159 – 161

3

Beta strand

179 – 182

4

Turn

184 – 187

4

Helix

190 – 200

11

Sequences

	Sequence		Length	Mass (Da)
	Q87WW0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C34C7ECA9448552F	FASTA	305	35,312
	10 20 30 40 50 60 MVDKLTHLKQ LEAESIHIIR EVAAEFDNPV MLYSIGKDSA VMLHLARKAF FPGKLPFPVM 70 80 90 100 110 120 HVDTRWKFQE MYRFRDQMVE EMGLDLITHI NPDGVAQGIN PFTHGSAKHT DIMKTEGLKQ 130 140 150 160 170 180 ALDKHGFDAA FGGARRDEEK SRAKERVYSF RDSKHRWDPK NQRPELWNVY NGNVNKGESI 190 200 210 220 230 240 RVFPLSNWTE LDIWQYIYLE GIPIVPLYFA AERDVIEKNG TLIMIDDERI LEHLTDEEKS 250 260 270 280 290 300 RIVKKKVRFR TLGCYPLTGA VESEATSLTD IIQEMLLTRT SERQGRVIDH DGAGSMEEKK RQGYF

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016853 Genomic DNA. Translation: AAO57882.1.

RefSeq

NP_794187.1. NC_004578.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUN	X-ray	2.70	A	1-305	[»]

ProteinModelPortal

Q87WW0.

SMR

Q87WW0. Positions 1-211.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1186114.

GenomeReviews

Gene locus PSPTO_4433 in contig AE016853_GR.

KEGG

pst:PSPTO_4433.

PATRIC

20000376. VBIPseSyr93040_4548.

TIGR

PSPTO_4433.

Phylogenomic databases

HOGENOM

HBG758022.

OMA

NARIHPG.

ProtClustDB

PRK05253.

Enzyme and pathway databases

BioCyc

PSYR223283:PSPTO_4433-MONOMER.

Family and domain databases

HAMAP

MF_00064. Sulf_adenylyltr_sub2.
[Tree]

InterPro

IPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.

KO

K00957.

Pfam

PF01507. PAPS_reduct. 1 hit.
[Graphical view]

PIRSF

PIRSF002936. CysDAde_trans. 1 hit.

TIGRFAMs

TIGR02039. CysD. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CYSD_PSESM

Accession

Primary (citable) accession number: Q87WW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program