Q87WD5 (ENO2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase 2
EC=4.2.1.11

Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2
2-phosphoglycerate dehydratase 2

Gene names

Name:	eno2
Synonyms:	eno-2
Ordered Locus Names:	PSPTO_4616

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis By similarity. HAMAP MF_00318
Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O. HAMAP MF_00318
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Enzyme regulation	The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein By similarity. HAMAP MF_00318
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. HAMAP MF_00318
Subcellular location	Cytoplasm. Secreted. Cell surface. Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial cell surface By similarity. HAMAP MF_00318
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm Secreted
Ligand	Magnesium Metal-binding
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Enolase 2 HAMAP MF_00318		PRO_0000133952
Regions
	Region	366 – 369	4	Substrate binding By similarity
Sites
	Active site	207	1	Proton donor By similarity
	Active site	339	1	Proton acceptor By similarity
	Metal binding	244	1	Magnesium By similarity
	Metal binding	287	1	Magnesium By similarity
	Metal binding	314	1	Magnesium By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity
	Binding site	314	1	Substrate By similarity
	Binding site	339	1	Substrate (covalent); in inhibited form By similarity
	Binding site	390	1	Substrate By similarity
Amino acid modifications
	Modified residue	281	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87WD5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 49D061CB165A93F2	FASTA	427	46,023
	10 20 30 40 50 60 MKTQIQAIHA REILDSRGNP TVEVDVTLEC GAMGRASVPS GASTGAHEAV ELRDKDTQRY 70 80 90 100 110 120 SGKGVLKAVS NVNTEILESV RGMNAIDQEQ IDHLMIKLDG TSDKSRLGGN AILGVSLAVA 130 140 150 160 170 180 RAAASALNLP LFQYLGGEQA ARMPVPMFNI LNGGVHANWQ GPDFQEFMIA PTGAGSFKEA 190 200 210 220 230 240 LRWGSEVYHE LKAVLKDAGY STAVGDEGGF APALKKNSDA IELIIKAIER AGYTPGSQIE 250 260 270 280 290 300 IAIDPASSGF YENGLYHLRS EGRKVDAQEL INLYSSWVDK YPIAVLEDGL AEDDWSGWKL 310 320 330 340 350 360 LNAALGDRIE LVGDDLFVTN VERIQRGITE NVANAVLIKP NQIGTLTETK AAIEMAYGAN 370 380 390 400 410 420 WGAMVSHRSG ETVDSSIADL TVAMGTGHLK TGAPCRGERV EKYNQFLRIE EDLGSRAFYA GHDAFVR

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO58062.1.
RefSeq	NP_794367.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q87WD5.
SMR	Q87WD5. Positions 3-427.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1186299.
GenomeReviews	Gene locus PSPTO_4616 in contig AE016853_GR.
KEGG	pst:PSPTO_4616.
NMPDR	fig|223283.1.peg.4505.
PATRIC	20000744. VBIPseSyr93040_4730.
TIGR	PSPTO_4616.
Phylogenomic databases
HOGENOM	HBG726599.
OMA	TNVERIQ.
ProtClustDB	PRK00077.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_4616-MONOMER.
Family and domain databases
HAMAP	MF_00318. Enolase. [Tree]
InterPro	IPR000941. Enolase. IPR020810. Enolase_C. IPR020809. Enolase_CS. IPR020811. Enolase_N. [Graphical view]
KO	K01689.
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
TIGRFAMs	TIGR01060. Eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENO2_PSESM

Accession

Primary (citable) accession number: Q87WD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program