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Q87VR8 (PUR2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PSPTO_4867
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151471

Regions

Domain108 – 315208ATP-grasp
Nucleotide binding134 – 19562ATP By similarity

Sites

Metal binding2851Magnesium or manganese By similarity
Metal binding2871Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87VR8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 17F1464E482C90AA

FASTA43145,523
        10         20         30         40         50         60 
MNVLIIGSGG REHALAWKVA QDPRVQKVFV APGNAGTAIE AKCENVAIDV LALEQLADFA 

        70         80         90        100        110        120 
ENNVSLTIVG PEVPLVAGVV DLFRSRGLDC FGPTAGAAQL EGSKAFTKDF LARHKIPTAD 

       130        140        150        160        170        180 
YQNFTEIEPA LAYLREKGAP IVIKADGLAA GKGVIVAMTL TEAEDAVQDM LAGNAFGEAG 

       190        200        210        220        230        240 
SRVVIEEFLD GEEASFIVMV DGKNVLPMAT SQDHKRVGDG DSGPNTGGMG AYSPAPVVTA 

       250        260        270        280        290        300 
DVHQRVMDLV IWPTVRGMAD EGNVYTGFLY AGLMIDKAGN PKVIEFNCRF GDPETQPVML 

       310        320        330        340        350        360 
RLQSSLVLLI EAALAQALDK VEAQWDPRPS LGIVLAAGGY PGDYAKGAII EGLDAAALLE 

       370        380        390        400        410        420 
GKIFHAGTTL QDDRVVTSGG RVLCATALGD SVGSAQKNAY ALAAKVDWQG CFYRTDIGYR 

       430 
AIARERGEDQ E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016853 Genomic DNA. Translation: AAO58296.1.
RefSeqNP_794601.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ87VR8.
SMRQ87VR8. Positions 1-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223283.PSPTO_4867.

Proteomic databases

PRIDEQ87VR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO58296; AAO58296; PSPTO_4867.
GeneID1186550.
KEGGpst:PSPTO_4867.
PATRIC20001242. VBIPseSyr93040_4979.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-4933-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PSESM
AccessionPrimary (citable) accession number: Q87VR8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways