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Q87VA4

- GSHB_PSESM

UniProt

Q87VA4 - GSHB_PSESM

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Protein

Glutathione synthetase

Gene
gshB, PSPTO_5035
Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi282 – 2821Magnesium or manganese By similarity
Metal bindingi284 – 2841Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 20858ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-5103-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshB
Ordered Locus Names:PSPTO_5035
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Glutathione synthetaseUniRule annotationPRO_0000197478Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_5035.

Structurei

3D structure databases

ProteinModelPortaliQ87VA4.
SMRiQ87VA4. Positions 3-315.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 311187ATP-graspAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiDWKIARA.
OrthoDBiEOG6WMHWB.
PhylomeDBiQ87VA4.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q87VA4-1 [UniParc]FASTAAdd to Basket

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MSVRLGIVMD PIERISYKKD SSLAMLLAAQ DRGWTLFYME QKDLYQNAGQ    50
ARARMKPLKV FADPAKWFEF EAEIDAGLDD LDVILMRKDP PFDMEFIYTT 100
YLLEQAESAG VLVVNKPQSL RDCNEKLFAT LFPQCTPPTL VSRRADILRE 150
FAEQQGDVIL KPLDGMGGAS IFRHRAGDPN LSVILETLTA HGTQQIMAQG 200
YLPAIKDGDK RILMVDGEPV PYCLARIPAA GETRGNLAAG GRGEARPLSD 250
KDRWIAEQIG PTLREKGLLF VGLDVIGEHL TEINVTSPTC IREIDNAFGT 300
NIGGLLMDAI EKKLQARKG 319
Length:319
Mass (Da):35,337
Last modified:June 1, 2003 - v1
Checksum:iCE851040931714C7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016853 Genomic DNA. Translation: AAO58463.1.
RefSeqiNP_794768.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO58463; AAO58463; PSPTO_5035.
GeneIDi1186720.
KEGGipst:PSPTO_5035.
PATRICi20001596. VBIPseSyr93040_5154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016853 Genomic DNA. Translation: AAO58463.1 .
RefSeqi NP_794768.1. NC_004578.1.

3D structure databases

ProteinModelPortali Q87VA4.
SMRi Q87VA4. Positions 3-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223283.PSPTO_5035.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO58463 ; AAO58463 ; PSPTO_5035 .
GeneIDi 1186720.
KEGGi pst:PSPTO_5035.
PATRICi 20001596. VBIPseSyr93040_5154.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000265022.
KOi K01920.
OMAi DWKIARA.
OrthoDBi EOG6WMHWB.
PhylomeDBi Q87VA4.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci PSYR223283:GJIX-5103-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00162. GSH_S.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01380. glut_syn. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiGSHB_PSESM
AccessioniPrimary (citable) accession number: Q87VA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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