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Protein

Thymidylate synthase

Gene

thyA

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei192 – 1921UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-5350-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PSPTO_5282
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Thymidylate synthasePRO_0000141006Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi223283.PSPTO_5282.

Structurei

3D structure databases

ProteinModelPortaliQ87UL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiQ87UL4.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87UL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLELVAH VIKHGTLQAN RTGVNTISFP GAMLRYDLQE GFPAITTRRM
60 70 80 90 100
AFKSAIGEMV GFLRGVSNAA EFRELGCKVW DQNANENAQW LNNPFRKGED
110 120 130 140 150
DLGEIYGVQW RKWPAYKRID TGNVAAIELA LGQGYRQIAE SEEDGQSFVV
160 170 180 190 200
LYKAIDQIRQ CIDTIINDPG SRRILFHGWN CAQLDEMALP PCHLLYQLHP
210 220 230 240 250
NPQTREISLT LYIRSNDLGL GTPFNLTEGA ALLSLIGRLT GYTPRWFTYF
260 270 280 290 300
IGDAHVYENH LDMLNEQMTR EPYPMPKLVI SDRVPEFAKT GVYQPEWLEL
310 320
IEPSDFSLEG YQHHPAMTAP MAV
Length:323
Mass (Da):36,724
Last modified:June 1, 2003 - v1
Checksum:i583D8B957E1055EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO58708.1.
RefSeqiNP_795013.1. NC_004578.1.
WP_011105406.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO58708; AAO58708; PSPTO_5282.
GeneIDi1186967.
KEGGipst:PSPTO_5282.
PATRICi20002102. VBIPseSyr93040_5407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA. Translation: AAO58708.1.
RefSeqiNP_795013.1. NC_004578.1.
WP_011105406.1. NC_004578.1.

3D structure databases

ProteinModelPortaliQ87UL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_5282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO58708; AAO58708; PSPTO_5282.
GeneIDi1186967.
KEGGipst:PSPTO_5282.
PATRICi20002102. VBIPseSyr93040_5407.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiQ87UL4.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciPSYR223283:GJIX-5350-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.

Entry informationi

Entry nameiTYSY_PSESM
AccessioniPrimary (citable) accession number: Q87UL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.