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Q87UL4 (TYSY_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:PSPTO_5282
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141006

Sites

Active site1921 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87UL4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 583D8B957E1055EE

FASTA32336,724
        10         20         30         40         50         60 
MKQYLELVAH VIKHGTLQAN RTGVNTISFP GAMLRYDLQE GFPAITTRRM AFKSAIGEMV 

        70         80         90        100        110        120 
GFLRGVSNAA EFRELGCKVW DQNANENAQW LNNPFRKGED DLGEIYGVQW RKWPAYKRID 

       130        140        150        160        170        180 
TGNVAAIELA LGQGYRQIAE SEEDGQSFVV LYKAIDQIRQ CIDTIINDPG SRRILFHGWN 

       190        200        210        220        230        240 
CAQLDEMALP PCHLLYQLHP NPQTREISLT LYIRSNDLGL GTPFNLTEGA ALLSLIGRLT 

       250        260        270        280        290        300 
GYTPRWFTYF IGDAHVYENH LDMLNEQMTR EPYPMPKLVI SDRVPEFAKT GVYQPEWLEL 

       310        320 
IEPSDFSLEG YQHHPAMTAP MAV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016853 Genomic DNA. Translation: AAO58708.1.
RefSeqNP_795013.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ87UL4.
ModBaseSearch...

Protein-protein interaction databases

STRING223283.PSPTO_5282.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO58708; AAO58708; PSPTO_5282.
GeneID1186967.
KEGGpst:PSPTO_5282.
PATRIC20002102. VBIPseSyr93040_5407.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257900.
KOK00560.
OMADTNVAYL.
ProtClustDBPRK13821.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-5350-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_PSESM
AccessionPrimary (citable) accession number: Q87UL4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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