Reviewed, UniProtKB/Swiss-Prot Q87U40 (UBIC_PSESM)

Names and origin

Protein names

Recommended name:
    Probable chorismate--pyruvate lyase
      Short name=CL
      Short name=CPL
    EC=4.1.3.40

Gene names

Name:	ubiC
Ordered Locus Names:	PSPTO_5475

Organism

Pseudomonas syringae pv. tomato [Complete proteome] [HAMAP]

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway By similarity. HAMAP MF_01632
Catalytic activity	Chorismate = 4-hydroxybenzoate + pyruvate. HAMAP MF_01632
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_01632
Subcellular location	Cytoplasm By similarity HAMAP MF_01632.
Sequence similarities	Belongs to the ubiC family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Cellular component	Cytoplasm
Ligand	Pyruvate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyruvate biosynthetic process Inferred from electronic annotation. Source: HAMAP ubiquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chorismate lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Probable chorismate--pyruvate lyase HAMAP MF_01632		PRO_0000240563
Sites
	Binding site	80	1	Substrate By similarity
	Binding site	118	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87U40-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 33689F58FAE72D19	FASTA	186	20,730
	10 20 30 40 50 60 MTQHSPAFVT PDWLEREQLT DTPTPVFFGW LFNQQSLTRR LDRLSDGGFD IVPLSEGWQA 70 80 90 100 110 120 LRDDECAALG LPAASEGWVR EVYLRGNGEN WVFARSVAAR DALQHGGLHM DELGTRSLGA 130 140 150 160 170 180 LLFSDPAFDR GTLQVCHYPQ SWLPDADAVS GLWARRSQFS RGALSVLVAE VFLPALWNAI HNKDHA

References

[1]

"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed: 12928499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016853 Genomic DNA. Translation: AAO58894.1.
RefSeq	NP_795199.1.
3D structure databases
SMR	Q87U40. Positions 5-176.
ModBase	Search...
Genome annotation databases
GeneID	1187167.
GenomeReviews	Gene locus PSPTO_5475 in contig AE016853_GR.
KEGG	pst:PSPTO_5475.
NMPDR	fig|223283.1.peg.5337.
TIGR	PSPTO_5475.
Phylogenomic databases
HOGENOM	HBG644467.
OMA	PVVFAHS.
Enzyme and pathway databases
BioCyc	PSYR223283:PSPTO_5475-MONOMER.
BRENDA	4.1.3.40. 289554.
Family and domain databases
HAMAP	MF_01632. UbiC. [Tree]
InterPro	IPR007440. Chorismate--pyruvate_lyase. [Graphical view]
Pfam	PF04345. Chor_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIC_PSESM

Accession

Primary (citable) accession number: Q87U40

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)