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Protein

Coenzyme A biosynthesis bifunctional protein CoaBC

Gene

coaBC

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine (By similarity).By similarity

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126SubstrateBy similarity1
Active sitei159Proton donorBy similarity1
Binding sitei279CTPBy similarity1
Binding sitei289CTPBy similarity1
Binding sitei340CTPBy similarity1
Binding sitei344CTPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase
LigandFlavoprotein, FMN, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
UPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme A biosynthesis bifunctional protein CoaBC
Alternative name(s):
DNA/pantothenate metabolism flavoprotein
Including the following 2 domains:
Phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Short name:
PPCDC
Alternative name(s):
CoaC
Phosphopantothenate--cysteine ligase (EC:6.3.2.5)
Alternative name(s):
CoaB
PPC synthetase
Short name:
PPCS
Phosphopantothenoylcysteine synthase
Gene namesi
Name:coaBC
Ordered Locus Names:VP0181
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820261 – 399Coenzyme A biosynthesis bifunctional protein CoaBCAdd BLAST399

Interactioni

Subunit structurei

Homododecamer, the CoaB domains form homodimers.By similarity

Protein-protein interaction databases

STRINGi223926.VP0181.

Structurei

3D structure databases

ProteinModelPortaliQ87T89.
SMRiQ87T89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 188Phosphopantothenoylcysteine decarboxylaseAdd BLAST188
Regioni189 – 399Phosphopantothenate--cysteine ligaseAdd BLAST211

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Curated
In the C-terminal section; belongs to the PPC synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJS. Bacteria.
COG0452. LUCA.
HOGENOMiHOG000037526.
KOiK13038.
OMAiVRFIGNH.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
3.40.50.1950. 1 hit.
InterProiView protein in InterPro
IPR035929. CoaB-like_sf.
IPR005252. CoaBC.
IPR007085. DNA/pantothenate-metab_flavo_C.
IPR036551. Flavin_trans-like.
IPR003382. Flavoprotein.
PfamiView protein in Pfam
PF04127. DFP. 1 hit.
PF02441. Flavoprotein. 1 hit.
SUPFAMiSSF102645. SSF102645. 1 hit.
SSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00521. coaBC_dfp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87T89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTLAGKKIL LGISGGIAAY KCAELTRRLI ERGAQVQVVM TKAAKEFITP
60 70 80 90 100
LTMQAVSGRP VSDSLLDPAA EASMGHIELA KWADLVLLAP ATADLIARMS
110 120 130 140 150
AGMGNDLLTT LVLATDSPVA VSPAMNQQMY RNIATQENIA TLARRGMNIW
160 170 180 190 200
GPAAGEQACG DVGPGRMLEP MQLVHLCEQF FQPKVLEGKS ILISAGPTRE
210 220 230 240 250
AIDPVRYITN HSSGKMGYAL ANAAAQLGAK VTLVSGPVNL STPMGVERIN
260 270 280 290 300
VSSAQEMYEA VMAQAISHDA FISCAAVADY RPEAIASQKL KKTADNDQMT
310 320 330 340 350
IKMVKNPDIV ASVAALTDKR PFTVGFAAET NDVETYARGK LAKKNLNMIC
360 370 380 390
ANDVSVEGQG FNSNDNAITL FWPDGELALA LESKEALSFK ILEKMRELM
Length:399
Mass (Da):42,628
Last modified:June 1, 2003 - v1
Checksum:i33D5C1A734805222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58444.1.
RefSeqiNP_796560.1. NC_004603.1.
WP_005478613.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC58444; BAC58444; BAC58444.
GeneIDi1187648.
KEGGivpa:VP0181.
PATRICifig|223926.6.peg.174.

Similar proteinsi

Entry informationi

Entry nameiCOABC_VIBPA
AccessioniPrimary (citable) accession number: Q87T89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families