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Protein

Adenylyl-sulfate kinase

Gene

cysC

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of activated sulfate.

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei113 – 1131Phosphoserine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-307-MONOMER.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinaseUniRule annotation (EC:2.7.1.25UniRule annotation)
Alternative name(s):
APS kinaseUniRule annotation
ATP adenosine-5'-phosphosulfate 3'-phosphotransferaseUniRule annotation
Adenosine-5'-phosphosulfate kinaseUniRule annotation
Gene namesi
Name:cysCUniRule annotation
Ordered Locus Names:VP0296
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Adenylyl-sulfate kinasePRO_0000105924Add
BLAST

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi223926.VP0296.

Structurei

3D structure databases

ProteinModelPortaliQ87SX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108I67. Bacteria.
COG0529. LUCA.
HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG6GR38Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87SX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAETPVKDE NIVWHQHTVD KQFRAELKKQ KPAVLWFTGL SGAGKSTVAG
60 70 80 90 100
ALENRLAELG YHTYLLDGDN VRHGLCSDLG FSEQDRRENI RRIGELAKLM
110 120 130 140 150
ADAGLIVLSA FISPHRAERQ LVRDLLPEGE FIEVFVNASL EVCEGRDPKG
160 170 180 190 200
LYKKARAGEI PNFTGIDSEY QAPINPEIDL PAGEKSVEEL VELCLNELKQ

RRVIS
Length:205
Mass (Da):22,894
Last modified:May 23, 2003 - v1
Checksum:i03696F141F48199F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58559.1.
RefSeqiNP_796675.1. NC_004603.1.
WP_005454655.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC58559; BAC58559; BAC58559.
GeneIDi1187763.
KEGGivpa:VP0296.
PATRICi20138822. VBIVibPar50997_0286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58559.1.
RefSeqiNP_796675.1. NC_004603.1.
WP_005454655.1. NC_004603.1.

3D structure databases

ProteinModelPortaliQ87SX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP0296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC58559; BAC58559; BAC58559.
GeneIDi1187763.
KEGGivpa:VP0296.
PATRICi20138822. VBIVibPar50997_0286.

Phylogenomic databases

eggNOGiENOG4108I67. Bacteria.
COG0529. LUCA.
HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG6GR38Q.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00205.
BioCyciVPAR223926:GHK4-307-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.

Entry informationi

Entry nameiCYSC_VIBPA
AccessioniPrimary (citable) accession number: Q87SX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: May 23, 2003
Last modified: May 11, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.