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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei605 – 6051For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-370-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:VP0359
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002493: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 610609Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135410Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi223926.VP0359.

Structurei

3D structure databases

ProteinModelPortaliQ87SR3.
SMRiQ87SR3. Positions 2-610.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 218217Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini286 – 426141SIS 1UniRule annotationAdd
BLAST
Domaini459 – 600142SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q87SR3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIVGAVAQ RDVAEILVEG LRRLEYRGYD SAGVAIVDAE ANLTRIRRLG
60 70 80 90 100
KVQELADAVD EAKVVGGTGI AHTRWATHGE PSEINAHPHM SGDITVVHNG
110 120 130 140 150
IIENHEELRE LLQSRGYVFE SQTDTEVIAH MVEWELRTAE SLLEAVQKTA
160 170 180 190 200
KQLEGAYGTV AMDRKDPSRI VVARSGSPIV IGFGVGENFL ASDQLALLNV
210 220 230 240 250
TRRFMYLEEG DVAEITRRDV TVFDVTGERV EREITESNAE HDAGDKGQYR
260 270 280 290 300
HFMQKEIYEQ PKALINTMEG RITADSVVTD AIGVHAADIL SKVEHVQIVA
310 320 330 340 350
CGTSYNAGMT ARYWFEDIAG VSCDVEIASE FRYRKFVTRP NSLLITLSQS
360 370 380 390 400
GETADTLAAL RLAKEKGYMA AMTICNVAGS SLVRESDFAF MTRAGVEIGV
410 420 430 440 450
ASTKAFTTQL SALLMLVTAL GKEQGRISKE KEKEIVEALH ALPKQINAAL
460 470 480 490 500
SFEKEIEALA TDFADKHHTL FLGRGEFYPI AMEASLKLKE ISYIHAEAYA
510 520 530 540 550
AGELKHGPLA LIDADMPVVV VAPSNDLLEK LKSNVEEVRA RGGLLYVFAD
560 570 580 590 600
ADAGFEGDET MKIITMPHVS EITAAIYYTI PMQLLSYYVA LIKGTDVDQP
610
RNLAKAVTVE
Length:610
Mass (Da):66,841
Last modified:January 23, 2007 - v2
Checksum:iDF344D6C374E9E60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58622.1.
RefSeqiNP_796738.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC58622; BAC58622; BAC58622.
GeneIDi1187826.
KEGGivpa:VP0359.
PATRICi20138940. VBIVibPar50997_0345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58622.1.
RefSeqiNP_796738.1. NC_004603.1.

3D structure databases

ProteinModelPortaliQ87SR3.
SMRiQ87SR3. Positions 2-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP0359.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC58622; BAC58622; BAC58622.
GeneIDi1187826.
KEGGivpa:VP0359.
PATRICi20138940. VBIVibPar50997_0345.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-370-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.

Entry informationi

Entry nameiGLMS_VIBPA
AccessioniPrimary (citable) accession number: Q87SR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.