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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:VP0423
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092821 – 947Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST947

Interactioni

Protein-protein interaction databases

STRINGi223926.VP0423.

Structurei

3D structure databases

ProteinModelPortaliQ87SK0.
SMRiQ87SK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 443Adenylyl removaseUniRule annotationAdd BLAST443
Regioni451 – 947Adenylyl transferaseUniRule annotationAdd BLAST497

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q87SK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLPSSLVSV AESAVQNAQE AGYLQSWPNE VVEQFHYVSA LSQFITETIH
60 70 80 90 100
RDEALAQQLP TMLSELSRHQ AYRTRLAALL AECPDEMSGH RVLRQFRNRE
110 120 130 140 150
MVYIAWKDFL HAWTLEESLR HLSQLAEAMI FETYQWQYKI CCAEWGTPTN
160 170 180 190 200
AEGEAQPMLI IGMGKLGGGE LNFSSDIDLI FTYPENGETQ GARRSIANAQ
210 220 230 240 250
FFTRLGQRII KALDQQTFDG FCYRVDMRLR PFGESGPLVM SYAALEDYYQ
260 270 280 290 300
EQGRDWERYA MIKARVMGCE MYPQYQELRK MLRPFVFRRY IDFSAIQSLR
310 320 330 340 350
RMKSMISSEV RRRGLTNNIK LGAGGIREIE FIAQVFQLIR GGREPSLRNR
360 370 380 390 400
GLLETLSGIE ELALLTPQEV SNLEAAYKYL RQLENLLQAM ADKQTQTLPD
410 420 430 440 450
CDIERLKLAT AMQLESWDLL IEQTQQHMNK VHQVFETLIG DDEEDEGSTI
460 470 480 490 500
ARHFHELWDM ANKQDVLELI LEQDIQVEEP AIFSKAIINF KADLAKKTLG
510 520 530 540 550
PRGREVLNRL MPKVFDAVFA HPDAQFGLPR VLHLLHNICT RTTYLELLDE
560 570 580 590 600
HPAALVQLVR LCTASPMISE QLSRYPILLD ELIDPQQLYN PIPLDSYRTE
610 620 630 640 650
LRDFLARIPE DDMEQQMEAL RQFKQICILR IAAADIAGVL PVMKVSDHLT
660 670 680 690 700
YLAEAIVEAV VSQAWLQVSE KYGEPTHVKD REGKGFAVIG YGKVGGWELG
710 720 730 740 750
YNSDLDIVFM HDCPVNVYTD GKKEIDGRQF YLRLAQRIIH IFSTRTASGI
760 770 780 790 800
LYEVDTRLRP SGASGLLVSP TDAFDDYQHQ DAWTWEHQAL VRARMIYGDE
810 820 830 840 850
PLAIAFHNTR HDVLCKPRDE QTLKKEVVEM REKMRDHLGG KKSGRFMIKQ
860 870 880 890 900
DVGGITDIEF LAQYLVLNYS HEKPKLTRWC DNVRIYETLI AQGVMEEDQA
910 920 930 940
MQLIRAYTAM RNEIHHRNLL NLDADVVEDK FVAEREWVKQ AWNQWFA
Length:947
Mass (Da):109,195
Last modified:June 1, 2003 - v1
Checksum:i4854E2D668AC5DE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58686.1.
RefSeqiNP_796802.1. NC_004603.1.
WP_005455447.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC58686; BAC58686; BAC58686.
GeneIDi1187891.
KEGGivpa:VP0423.
PATRICi20139056. VBIVibPar50997_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC58686.1.
RefSeqiNP_796802.1. NC_004603.1.
WP_005455447.1. NC_004603.1.

3D structure databases

ProteinModelPortaliQ87SK0.
SMRiQ87SK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP0423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC58686; BAC58686; BAC58686.
GeneIDi1187891.
KEGGivpa:VP0423.
PATRICi20139056. VBIVibPar50997_0402.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_VIBPA
AccessioniPrimary (citable) accession number: Q87SK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2003
Last modified: May 10, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.