Q87RW0 (PUR4_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	VP0666

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1302	1302	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100421
Regions
	Domain	1049 – 1302	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1142	1	Nucleophile By similarity
	Active site	1267	1	By similarity
	Active site	1269	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87RW0 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 00C5C7FD41595FB1	FASTA	1,302	141,687
	10 20 30 40 50 60 MRILRGSPAL SEFRVNKLLE LCREQDLPVT GIYAEFMHFA DLKSDLDDQE LEKLEKLLTY 70 80 90 100 110 120 GPTIEEHEPE GLLLLVTPRP GTISPWSSKS TDIAINCGLD TVKRLERGTA YYVESSVVLS 130 140 150 160 170 180 EAQVDAVKAL IHDRMMETVF TELEAASALF TVAEPKPVAH VDILAGGRLA LEEANVSLGL 190 200 210 220 230 240 ALAEDEIDYL VENFTKLGRN PNDIELMMFA QANSEHCRHK IFNADWTIDG VDQEKSLFKM 250 260 270 280 290 300 IKNTFETTPD HVLSAYKDNA AVMTGSKVGR FFPDPKSRQY TYHHEDAHIL MKVETHNHPT 310 320 330 340 350 360 AISPWPGAST GSGGEIRDEG ATGIGGKPKA GLVGFTTSNL RIPGFEQPWE TDFGKPGRIV 370 380 390 400 410 420 NALDIMLEGP LGGAAFNNEF GRPNLLGYFR TYEEKVTSHA GEEVRGYHKP IMIAGGMGNI 430 440 450 460 470 480 RDEHVQKKEI PVGASLIVLG GPAMNIGLGG GAASSMASGQ SAEDLDFASV QRENPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGEENPIAFI HDVGAGGISN ALPELCDDGE RGGKFQLRDV PNDELSMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAPENM EAFDAICKRE RAPYAVVGVA TEERHLTLED SHFDNTPIDM 610 620 630 640 650 660 PMDILLGKTP KMHREATTLK VDSPAIARDG IEIDEAADRV LRLPTVAEKT FLITIGDRSV 670 680 690 700 710 720 TGLVARDQMV GPWQVPVANC AVTAASYDTY HGEAMSMGER TPVALLDFGA SARLAVGESL 730 740 750 760 770 780 TNIAATDIGD IKRIKLSANW MSPAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 790 800 810 820 830 840 MKTKWEENGE SKEVTSPLSL VITAFGRVED VRKTVTPQLR TSDTLEGLGD TSLVLVDLGN 850 860 870 880 890 900 GKNRLGATAL AQVYKQLGDK PADVDNAEQL KGFFDAMQNL VRNDKLLAYH DKGDGGLFVT 910 920 930 940 950 960 LAEMAFAGHC GVKADIAELG EDALAVLFNE ELGAVVQVKN DDLDSVLSTL AANGLEACSH 970 980 990 1000 1010 1020 VIGSVEASDD FVFTSGDDVV LKRSRTELRV IWAETTHKMQ ALRDNPACAD QEFEAKKDNT 1030 1040 1050 1060 1070 1080 DPGLNVSLSF DVNEDIAAPY IAKGAKPKMA ILREQGVNSH VEMAAAFDRA GFEATDIHMS 1090 1100 1110 1120 1130 1140 DILTGQAVLD EYQGLVACGG FSYGDVLGAG EGWAKSILFN AQAREQFQAF FNREETFSLG 1150 1160 1170 1180 1190 1200 VCNGCQMLSN LKELIPGADL WPRFVRNESE RFEARFSLVE VQKSDSVFFD GMAGSRMPIA 1210 1220 1230 1240 1250 1260 VSHGEGRVEV RDGEHLNAIE ASGTVALRYV DNNGNPTQQY PNNPNGSPNA ITGLTTADGR 1270 1280 1290 1300 VTIMMPHPER VFRTVANSWA PEGWGENGAW MRMFQNARKN IG

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC58929.1.
RefSeq	NP_797045.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87RW0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1188141.
GenomeReviews	Gene locus VP0666 in contig BA000031_GR.
KEGG	vpa:VP0666.
NMPDR	fig|223926.1.peg.666.
PATRIC	20139534. VBIVibPar50997_0634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
OMA	VKAVGME.
ProtClustDB	PRK05297.
Enzyme and pathway databases
BioCyc	VPAR223926:VP0666-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR022940. PRibForGlyAmidine_synth_bac. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_VIBPA

Accession

Primary (citable) accession number: Q87RW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program