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Q87RQ0 (SYL_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:VP0727
OrganismVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) [Complete proteome] [HAMAP]
Taxonomic identifier223926 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length857 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 857857Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152114

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif617 – 6215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87RQ0 [UniParc].

Last modified May 23, 2003. Version 1.
Checksum: D84628AA1E5EE5EB

FASTA85796,654
        10         20         30         40         50         60 
MQEQYNPQDI EQKVQKHWDD NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRFQRLQG KNVMQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WNREFATCTP EYYRWEQEFF TKLYEKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITEYAQ ELLDDLDKLE GWPEMVKTMQ RNWIGRSEGV ELKFEVKGQQ 

       250        260        270        280        290        300 
DLEVYTTRPD TLMGVTYVGI AAGHPLATLA AENNPELAAF IEECKNTKVA EAELATMEKK 

       310        320        330        340        350        360 
GMATGLTAIH PLNGREVPVY VANFVLMDYG TGAVMAVPAH DQRDFEFATK YGLDIIPVIK 

       370        380        390        400        410        420 
PADGSELDIS EAAYTEKGVL FDSGEFDGLE FQAAFDAIAA KLEAEGKGTK TVNFRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGAP IPMVTTEDGE VHPVPADQLP VILPEDVVMD GVTSPIKADK EWAKTTFNGE 

       490        500        510        520        530        540 
PALRETDTFD TFMESSWYYA RYCSPQADDI LDPEKANYWL PVDQYIGGIE HACMHLLYSR 

       550        560        570        580        590        600 
FFHKLLRDAG YVTSDEPFKQ LLCQGMVLAD AFYFENEKGG KEWVAPTDVA VERDGKGRII 

       610        620        630        640        650        660 
SAKDNEGRDV THSGMIKMSK SKNNGIDPQE MVDKYGADTV RLFMMFASPA DMTLEWQESG 

       670        680        690        700        710        720 
VEGANRFLKR VWKLVKEHAE KGAAEAVDTA ALSGEQKALR RDVHKTIAKV TDDIARRQTF 

       730        740        750        760        770        780 
NTAIAAIMEL MNKLAKAPQE SAQDRAILDE ALKAVVTMLY PITPHISYEL WTALGESDID 

       790        800        810        820        830        840 
NAAWPTFDEK ALVEDEKTIV VQVNGKLRAK LTVAADATKE QVEELGLNDE NVTKFTDGLT 

       850 
IRKVIYVPGK LLNIVAN 

« Hide

References

[1]"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RIMD 2210633.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000031 Genomic DNA. Translation: BAC58990.1.
RefSeqNP_797106.1. NC_004603.1.

3D structure databases

ProteinModelPortalQ87RQ0.
SMRQ87RQ0. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223926.VP0727.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC58990; BAC58990; BAC58990.
GeneID1188202.
KEGGvpa:VP0727.
PATRIC20139658. VBIVibPar50997_0696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycVPAR223926:GHK4-746-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VIBPA
AccessionPrimary (citable) accession number: Q87RQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: May 23, 2003
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries