Reviewed, UniProtKB/Swiss-Prot Q87QI7 (TORA_VIBPA)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	VP1162

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 820	787	Trimethylamine-N-oxide reductase		PRO_0000019160

Sequences

	Sequence		Length	Mass (Da)
	Q87QI7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9D2E92390C187151	FASTA	820	92,542
	10 20 30 40 50 60 MAITRRSFLK GVATTSAASV IGPSLLASAS ANAAESTGTW KVTGSHWGAF RAHIYAGKVQ 70 80 90 100 110 120 EIKPLELDKN PTEMLNGIKG IIYSPSRVRY PMVRLDWLKK HKYSADTRGN NRFIRVTWDE 130 140 150 160 170 180 ALDLFYRELE RVQKEYGPWA LHAGQTGWNQ TGSFNNCTAH MQRAVGMHGN FITKVGDYST 190 200 210 220 230 240 GAGQTIMPYV LGSTEVYAQG TSWSEILENS DNIILWANDP VKNLQVGWNC ETHESFKYLA 250 260 270 280 290 300 ELKEKVAKGE INVLSVDPVK NKTQRYLEND HLYINPMTDV AFMLAVAHVL YNENLYDKKF 310 320 330 340 350 360 IDTYCLGFEE FIQYVQGKTK DKVEKTPEWA AAICGVKADK IREFARMLVS GRTQILMGWC 370 380 390 400 410 420 IQRQEHGEQP YWAAAVVAAM VGQIGLPGGG ISYGHHYSSI GVPSTGFAGP GGFPRNLDQG 430 440 450 460 470 480 MKPKWDNNDF NGYSRTIPVA RWIDCLLEPG KEINYNGGKV KLPDFKMMVI SGCNPWHHHQ 490 500 510 520 530 540 DRNRMKKAFR KLQTVVTIEF AWTATCRFSD IVLPACTQWE RNDIDVYGSY SNKGLIAMHR 550 560 570 580 590 600 LVDPLFQSKP DFQIMSELTQ RFGRREEYTR GMSEMEWIES LYNDCKKANE GKFEMPEFNE 610 620 630 640 650 660 FWEKSVLDFG EGKPWVRHAD FRKDPELNPL GTPSGFIEIT SRKIGRYGYE HCQEHPMWFE 670 680 690 700 710 720 KSERSHGGPG SDKYPFWLQS CHPDKRLHSQ MCESEEFRAT YAVQGREPVY INPIDAKAKG 730 740 750 760 770 780 IKDGDLVRVF NGRGQLLAGA VLTDSYPRGV IRIEEGAWYG PLNEKEGAIC TYGDPNTLTQ 790 800 810 820 DIGSSELAQA TSANTCIVDF EKFTGKVPPV TSFGGPIEVA

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC59425.1.
RefSeq	NP_797541.1.
3D structure databases
SMR	Q87QI7. Positions 40-819.
ModBase	Search...
Genome annotation databases
GeneID	1188667.
GenomeReviews	Gene locus VP1162 in contig BA000031_GR.
KEGG	vpa:VP1162.
NMPDR	fig|223926.1.peg.1162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304064.
OMA	NAETHES.
Enzyme and pathway databases
BioCyc	VPAR223926:VP1162-MONOMER.
BRENDA	1.7.2.3. 3063.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_VIBPA

Accession

Primary (citable) accession number: Q87QI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)