Q87QA3 (SERC_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine aminotransferase
EC=2.6.1.52

Alternative name(s):
Phosphohydroxythreonine aminotransferase
Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	VP1247

Organism

Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) [Complete proteome] [HAMAP]

Taxonomic identifier

223926 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio harveyi group › Vibrio parahaemolyticus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP-Rule MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP-Rule MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP-Rule MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP-Rule MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP-Rule MF_00160
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.
Sequence caution	The sequence BAC59510.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Phosphoserine aminotransferase HAMAP-Rule MF_00160		PRO_0000150216
Regions
	Region	80 – 81	2	Pyridoxal phosphate binding By similarity
	Region	241 – 242	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	46	1	L-glutamate By similarity
	Binding site	106	1	Pyridoxal phosphate By similarity
	Binding site	157	1	Pyridoxal phosphate By similarity
	Binding site	176	1	Pyridoxal phosphate By similarity
	Binding site	199	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	200	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87QA3 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: 617969D78437F489	FASTA	364	40,201
	10 20 30 40 50 60 MEQNTDNVFN FSAGPAALPK AVMQQAQQEL IDWQGLGTSV MEISHRSKEF IKVAQEAEQD 70 80 90 100 110 120 LRDLLNIPDN YKVLFCQGGA RAQFAAVPLN LLGDAETATY IDGGYWAESA VEEAKKYCEP 130 140 150 160 170 180 DVFDAKTEID GKVAVLPASE WKIAPEAAYV HFCPNETIDG IEINDLPITD KPIVADMSST 190 200 210 220 230 240 ILSREIDVSK YGVIYAGAQK NIGPSGIAIA IVRDDLLGMA KQVLPSILDY KVLAEKESMF 250 260 270 280 290 300 NTPPTFAWYL SGLVFKWLKA QGGVKSIETV NREKAKLLYD YIDQSDFYRN GVHPSNRSLM 310 320 330 340 350 360 NVPFQLAKPE LDATFLELAD AKGLKALKGH RVVGGMRASI YNAMPLEGVQ ALVDFMKEFE QNYA

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC59510.1. Different initiation.
RefSeq	NP_797626.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87QA3.
SMR	Q87QA3. Positions 8-364.
ModBase	Search...
Protein-protein interaction databases
STRING	223926.VP1247.
Proteomic databases
PRIDE	Q87QA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC59510; BAC59510; BAC59510.
GeneID	1188752.
KEGG	vpa:VP1247.
PATRIC	20140698. VBIVibPar50997_1186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1932.
HOGENOM	HOG000088965.
KO	K00831.
OMA	MSIMEMS.
ProtClustDB	PRK05355.
Enzyme and pathway databases
UniPathway	UPA00135; UER00197. UPA00244; UER00311.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_00160. SerC_aminotrans_5.
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR022278. Pser_aminoTfrase. IPR003248. Pser_aminoTfrase_subgr. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR21152:SF1. PTHR21152:SF1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000525. SerC. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_VIBPA

Accession

Primary (citable) accession number: Q87QA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program