ID   MAO1_VIBPA              Reviewed;         562 AA.
AC   Q87Q92;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=VP1258;
OS   Vibrio parahaemolyticus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633 / Serotype O3:K6;
RX   MEDLINE=22508454; PubMed=12620739; DOI=10.1016/S0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; BA000031; BAC59521.1; -; Genomic_DNA.
DR   RefSeq; NP_797637.1; -.
DR   HSSP; P40927; 1GQ2.
DR   GeneID; 1188763; -.
DR   GenomeReviews; BA000031_GR; VP1258.
DR   KEGG; vpa:VP1258; -.
DR   NMPDR; fig|223926.1.peg.1258; -.
DR   HOGENOM; Q87Q92; -.
DR   OMA; Q87Q92; IQDNNET.
DR   BioCyc; VPAR223926:VP1258-MON; -.
DR   BRENDA; 1.1.1.38; 3063.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_0000160236.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62299 MW;  E1B8D7A80CF073F3 CRC64;
     MNNDKRPLYI PYAGPALMAT PLLNKGSAFS AEERSSFNLE GLLPETTETI QEQVERAYQQ
     YKSFESDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR
     GLFISYPNRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG
     GISPAYTLPI VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DAFVEEFIQA VQRRWPDALI
     QFEDFAQKNA MPLLERYKDR ICCFNDDIQG TAAVTVGSLL AACKAAGTQL SKQRITFLGA
     GSAGCGIAEA IIAQMVSEGI SDEKARSQVY MVDRWGLLQE GMPNLLDFQQ RLVQKHSNTK
     EWENEGNGFS LLDVMRNAKP TVLIGVSGAP GLFSQEVIEE MHKHCKRPIV FPLSNPTSRV
     EATPNDIIRW TNGEALVATG SPFDPVVHEG RTYPIAQCNN SYIFPGIGLG VLAVNAKRVT
     DEMLMESSRA LATCSPLAIN GRGALLPPLE EIHLVSKKIA FAVAKKAIEQ GVALEITDEA
     LNDAIDQAFW QPVYRRYKRT AF
//